Arboleda P H, Loppnow G R
Department of Chemistry, University of Alberta, Edmonton, Canada.
Anal Chem. 2000 May 1;72(9):2093-8. doi: 10.1021/ac991389f.
Raman spectra of nine anomerically stable monosaccharides have been obtained in aqueous solution in the 700-1700 cm(-1) spectral range. Good-quality spectra are obtained of solutions with concentrations as low as 10 mM and volumes as small as 15 microL. Interestingly, the Raman spectra appear to be exquisitely sensitive to the configuration of the carbon centers; unique spectra are obtained of all nine monosaccharides. The unique Raman spectral fingerprint observed for each monosaccharide, and for each anomer of each monosaccharide, suggests that Raman spectroscopy may be a useful technique for the identification and characterization of biologically relevant oligosaccharides. To test this idea, Raman spectra of three unknown disaccharides were obtained in a single-blind study. Identification of the individual monosaccharide components and their anomeric configuration was completely successful. All of these results suggest that development of Raman spectroscopy as a fast, sensitive discovery tool in glycobiology and carbohydrate chemistry is straightforward.
在700 - 1700 cm(-1)光谱范围内，已获得九种端基异构稳定单糖在水溶液中的拉曼光谱。对于浓度低至10 mM且体积小至15 μL的溶液，也能获得高质量的光谱。有趣的是，拉曼光谱似乎对碳中心的构型极为敏感；所有九种单糖都能得到独特的光谱。观察到的每种单糖及其每个端基异构体的独特拉曼光谱指纹表明，拉曼光谱可能是一种用于鉴定和表征具有生物学相关性的寡糖的有用技术。为了验证这一想法，在一项单盲研究中获得了三种未知二糖的拉曼光谱。对各个单糖成分及其端基异构构型的鉴定完全成功。所有这些结果表明，将拉曼光谱发展成为糖生物学和碳水化合物化学中一种快速、灵敏的发现工具是可行的。
