Ryang D W, Koo S B, Shin M G, Shin J H, Suh S P
Department of Clinical Pathology, Chonnam University Medical School, Kwangju 501-757, Korea.
Jpn J Infect Dis. 1999 Apr;52(2):38-41.
Primary V. vulnificus septicemia has been continuously reported in Korea. We analyzed the molecular diversity of V. vulnificus strains isolated from clinical specimens using pulsed-field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) analysis. We analyzed a total of 23 V. vulnificus strains isolated from 22 patients between 1992 and 1997. RAPD analysis was performed using five random primers, and we obtained chromosomal DNA restriction patterns with NotI and SfiI by PFGE. Two isolates from one patient disclosed similarity value 1.0 by RAPD and had an indistinguishable pattern when analyzed with PFGE, which indicated that they were the same strains. The remaining 22 isolates from 22 patients could be separated into 5 different molecular types in RAPD analysis. The range of similarity values among the isolates was wide (0.29-0.81). Of 22 strains, four strains could not be typed in repeated trials by PFGE with NotI and SfiI. The PFGE patterns of 18 strains showed a remarkable polymorphism consisting 12-19 fragments (20-870 kb). These results show that V. vulnificus strains isolated from Korea are genetically diversified for the most part.
韩国持续报告有原发性创伤弧菌败血症病例。我们使用脉冲场凝胶电泳（PFGE）和随机扩增多态性DNA（RAPD）分析，对从临床标本中分离出的创伤弧菌菌株的分子多样性进行了分析。我们共分析了1992年至1997年间从22例患者中分离出的23株创伤弧菌菌株。使用5种随机引物进行RAPD分析，并通过PFGE获得了用NotI和SfiI酶切产生的染色体DNA限制图谱。从一名患者身上分离出的两株菌株经RAPD分析相似度值为1.0，用PFGE分析时具有难以区分的图谱，这表明它们是同一菌株。在RAPD分析中，从22例患者身上分离出的其余22株菌株可分为5种不同的分子类型。这些菌株之间的相似度值范围很广（0.29 - 0.81）。在22株菌株中，有4株在用NotI和SfiI进行PFGE重复试验时无法分型。18株菌株的PFGE图谱显示出显著的多态性，由12 - 19个片段（20 - 870 kb）组成。这些结果表明，从韩国分离出的创伤弧菌菌株在很大程度上具有遗传多样性。
