直接基因组限制酶分析和脉冲场凝胶电泳用于创伤弧菌分型的比较及其与多位点序列分型数据的对应关系
Comparison of direct genome restriction enzyme analysis and pulsed-field gel electrophoresis for typing of Vibrio vulnificus and their correspondence with multilocus sequence typing data.
作者信息
González-Escalona Narjol, Whitney Brooke, Jaykus Lee-Ann, DePaola Angelo
机构信息
Department of Food Science, North Carolina State University, Raleigh, North Carolina, USA.
出版信息
Appl Environ Microbiol. 2007 Nov;73(22):7494-500. doi: 10.1128/AEM.00738-07. Epub 2007 Aug 24.
Abstract
We compared the potential of direct genome restriction enzyme analysis (DGREA) and pulsed-field gel electrophoresis (PFGE) for discriminating Vibrio vulnificus isolates from clinical (23) and environmental (17) sources. The genotypes generated by both methodologies were compared to previous multilocus sequence typing (MLST) data. DGREA established clearer relationships among V. vulnificus strains and was more consistent with MLST than with PFGE. DGREA is a very promising tool for epidemiological and ecological studies of V. vulnificus.
摘要
我们比较了直接基因组限制性内切酶分析（DGREA）和脉冲场凝胶电泳（PFGE）区分临床来源（23株）和环境来源（17株）创伤弧菌分离株的潜力。将两种方法产生的基因型与之前的多位点序列分型（MLST）数据进行比较。DGREA在创伤弧菌菌株之间建立了更清晰的关系，并且与MLST的一致性高于PFGE。DGREA是用于创伤弧菌流行病学和生态学研究的非常有前景的工具。
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