Heerschap A, Houtman C, in 't Zandt H J, van den Bergh A J, Wieringa B
Department of Radiology, Faculty of Medical Sciences, University of Nijmegen, The Netherlands.
Proc Nutr Soc. 1999 Nov;58(4):861-70. doi: 10.1017/s0029665199001160.
31P magnetic resonance spectroscopy (MRS) offers a unique non-invasive window on energy metabolism in skeletal muscle, with possibilities for longitudinal studies and of obtaining important bioenergetic data continuously and with sufficient time resolution during muscle exercise. The present paper provides an introductory overview of the current status of in vivo 31P MRS of skeletal muscle, focusing on human applications, but with some illustrative examples from studies on transgenic mice. Topics which are described in the present paper are the information content of the 31P magnetic resonance spectrum of skeletal muscle, some practical issues in the performance of this MRS methodology, related muscle biochemistry and the validity of interpreting results in terms of biochemical processes, the possibility of investigating reaction kinetics in vivo and some indications for fibre-type heterogeneity as seen in spectra obtained during exercise.
31P磁共振波谱（MRS）为骨骼肌能量代谢提供了一个独特的非侵入性观察窗口，具有进行纵向研究的可能性，并且能够在肌肉运动期间以足够的时间分辨率连续获取重要的生物能量数据。本文对骨骼肌活体31P MRS的现状进行了介绍性综述，重点关注人体应用，但也列举了一些转基因小鼠研究的示例。本文所描述的主题包括骨骼肌31P磁共振波谱的信息内容、该MRS方法实施中的一些实际问题、相关的肌肉生物化学以及根据生化过程解释结果的有效性、体内研究反应动力学的可能性以及运动期间获得的波谱中所见纤维类型异质性的一些迹象。
