Valkovič Ladislav, Chmelík Marek, Krššák Martin
High-field MR Centre, Department of Biomedical Imaging and Image-guided Therapy, Medical University of Vienna, Vienna, Austria; Oxford Centre for Clinical Magnetic Resonance Research (OCMR), University of Oxford, Oxford, United Kingdom; Department of Imaging Methods, Institute of Measurement Science, Slovak Academy of Sciences, Bratislava, Slovakia.
High-field MR Centre, Department of Biomedical Imaging and Image-guided Therapy, Medical University of Vienna, Vienna, Austria; Christian Doppler Laboratory for Clinical Molecular MR Imaging, Vienna, Austria; Institute for Clinical Molecular MRI in Musculoskeletal System, Karl Landsteiner Society, Vienna, Austria.
Anal Biochem. 2017 Jul 15;529:193-215. doi: 10.1016/j.ab.2017.01.018. Epub 2017 Jan 21.
In addition to direct assessment of high energy phosphorus containing metabolite content within tissues, phosphorus magnetic resonance spectroscopy (P-MRS) provides options to measure phospholipid metabolites and cellular pH, as well as the kinetics of chemical reactions of energy metabolism in vivo. Even though the great potential of P-MR was recognized over 30 years ago, modern MR systems, as well as new, dedicated hardware and measurement techniques provide further opportunities for research of human biochemistry. This paper presents a methodological overview of the P-MR techniques that can be used for basic, physiological, or clinical research of human skeletal muscle and liver in vivo. Practical issues of P-MRS experiments and examples of potential applications are also provided. As signal localization is essential for liver P-MRS and is important for dynamic muscle examinations as well, typical localization strategies for P-MR are also described.
除了直接评估组织内含高能磷的代谢物含量外，磷磁共振波谱法（P-MRS）还提供了测量磷脂代谢物和细胞pH值的方法，以及体内能量代谢化学反应的动力学。尽管30多年前就认识到了P-MR的巨大潜力，但现代磁共振系统以及新的专用硬件和测量技术为人类生物化学研究提供了更多机会。本文介绍了可用于人体骨骼肌和肝脏体内基础、生理或临床研究的P-MR技术的方法概述。还提供了P-MRS实验的实际问题和潜在应用的示例。由于信号定位对肝脏P-MRS至关重要，对动态肌肉检查也很重要，因此还描述了P-MR的典型定位策略。
