Two-dimensional immunoelectrophoresis: a new simplified method.

A new method of two-dimensional immunoelectrophoresis is described, in which fixed amounts of both antigen (total proteins) and antibody are employed. When applied to normal human sera, it results in the appearance of 15 peaks accounting for the commonest serum proteins with the only exception of alpha 1-acid glycoprotein. Transferrin, measured by Laurell's or Mancini's technique, is used as an internal standard. The height of the transferrin peak at 300 mg% is determined by a calibration curve. The heights of the various peaks are thus expressed as percentages of that of transferrin at 300 mg%. The method appears to be simple, rapid and antiserum-saving.