Wesenberg F
Acta Pathol Microbiol Scand C. 1979 Aug;87C(4):301-6.
Eluates of 13 malignant and 17 normal tissues were prepared at 56 degrees C using the continuous flow technique. Albumin was detected in all the eluates. IgG, IgA, C3 or haptoglobin were detected in most of the malignant and some of the normal tissues. Carcinoembryonic antigen, beta 2-microglobulin, alpha 1-antitrypsin or alpha 1-antichymotrypsin were detected in some of the eluates of the malignant tissues only. IgM, IgD, C1q, C4, Cl-INH, alpha 1-macroglobulin, beta 2-lipoprotein, fibrinogen and alpha 1-foetoprotein were not detected in any of the eluates. The ratio of the concentration of albumin to the concentration of IgG was similar in extracts and eluates of all the normal tissue and in 3 of the malignant tissues indicating non-specific binding of IgG.
采用连续流动技术在56摄氏度下制备了13份恶性组织和17份正常组织的洗脱液。所有洗脱液中均检测到白蛋白。在大多数恶性组织和一些正常组织中检测到IgG、IgA、C3或触珠蛋白。仅在部分恶性组织洗脱液中检测到癌胚抗原、β2-微球蛋白、α1-抗胰蛋白酶或α1-抗糜蛋白酶。在任何洗脱液中均未检测到IgM、IgD、C1q、C4、Cl-INH、α1-巨球蛋白、β2-脂蛋白、纤维蛋白原和α1-甲胎蛋白。所有正常组织以及3份恶性组织的提取物和洗脱液中白蛋白浓度与IgG浓度之比相似，表明IgG存在非特异性结合。
