Resko J A, Pereyra-Martinez A C, Stadelman H L, Roselli C E
Department of Physiology and Pharmacology, School of Medicine, Oregon Health Sciences University, Portland, Oregon 97201-3098, USA.
Biol Reprod. 2000 Jun;62(6):1818-22. doi: 10.1095/biolreprod62.6.1818.
We demonstrated previously that testosterone regulates aromatase activity in the anterior/dorsolateral hypothalamus of male rhesus macaques. To determine the level of the androgen effect, we developed a ribonuclease protection assay to study the effects of testosterone or dihydrotestosterone (DHT) on aromatase (P450(AROM)) mRNA in selected brain areas. Adult male rhesus monkeys were treated with testosterone or DHT. Steroids in serum were quantified by RIA. Fourteen brain regions were analyzed for P450(AROM) mRNA. Significant elevations of its message over controls (P<0.05) were found in the medial preoptic area/anterior hypothalamus of both androgen treatment groups and the medial basal hypothalamus of the testosterone-treated males. Other brain areas were not affected by androgen treatment. We conclude that testosterone and DHT regulate P450(AROM) mRNA in brain regions that mediate reproductive behaviors and gonadotropin release. The P450(AROM) mRNA of other brain areas is not androgen dependent. Brain-derived estrogens may also be important for maintaining neural circuitry in brain areas not related to reproduction. The control of P450(AROM) mRNA in these areas may differ from what we report here, but it is equally important to understand the function of in situ estrogen formation in these areas.
我们之前证实，睾酮可调节雄性恒河猴前侧/背外侧下丘脑的芳香化酶活性。为确定雄激素作用的水平，我们开发了一种核糖核酸酶保护分析方法，以研究睾酮或双氢睾酮（DHT）对选定脑区芳香化酶（P450(AROM)）mRNA的影响。成年雄性恒河猴接受睾酮或DHT治疗。通过放射免疫分析法定量血清中的类固醇。对14个脑区进行P450(AROM) mRNA分析。在两个雄激素治疗组的内侧视前区/下丘脑前部以及睾酮治疗雄性的内侧基底下丘脑，发现其mRNA水平显著高于对照组（P<0.05）。其他脑区未受雄激素治疗影响。我们得出结论，睾酮和DHT调节介导生殖行为和促性腺激素释放的脑区中的P450(AROM) mRNA。其他脑区的P450(AROM) mRNA不依赖雄激素。脑源性雌激素对于维持与生殖无关的脑区的神经回路可能也很重要。这些区域中P450(AROM) mRNA的调控可能与我们在此报告的不同，但了解这些区域原位雌激素形成的功能同样重要。
