Choi E H, Lee H J
Immunocompromised Host Section, Pediatric Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.
J Infect Dis. 2000 May;181(5):1547-56. doi: 10.1086/315468. Epub 2000 May 15.
To study genetic variation and molecular epidemiology of the G protein of respiratory syncytial virus (RSV), 253 strains from a children's hospital in Korea over 9 consecutive epidemics were analyzed. Restriction analysis of the entire G protein gene demonstrated 24 genotypes among 188 subgroup A and 6 among 65 subgroup B isolates. Two to 4 dominant genotypes of subgroup A cocirculated, and different genotypes predominated in each epidemic. Predominant genotypes were replaced with new genotypes during consecutive epidemics. One of 2 dominant genotypes among subgroup B predominated alternately or concurrently. Phylogenetic analysis revealed that there were multiple lineages, with clustering related to their location and time of isolation among strains from Korea and worldwide. Geographic and temporal distinction have been shown more clearly for subgroup B than subgroup A. These results suggest that the G protein of RSV is continuously evolving, with a distinct pattern presumably due to immune selection in a localized region over time.
为研究呼吸道合胞病毒（RSV）G蛋白的基因变异和分子流行病学，对韩国一家儿童医院连续9次流行期间收集的253株病毒进行了分析。对整个G蛋白基因的限制性分析显示，188株A亚组病毒中有24种基因型，65株B亚组病毒中有6种基因型。A亚组有2至4种优势基因型共同流行，且每次流行中占主导地位的基因型不同。在连续的流行期间，优势基因型被新的基因型所取代。B亚组的2种优势基因型之一交替或同时占主导地位。系统发育分析表明，存在多个谱系，韩国和全球分离株的聚类与其分离地点和时间相关。B亚组的地理和时间差异比A亚组更明显。这些结果表明，RSV的G蛋白在持续进化，可能由于局部地区随时间的免疫选择而呈现出独特的模式。
