Andersson L C, Wasastjerna C, Gahmberg C G
Int J Cancer. 1976 Jan 15;17(1):40-6. doi: 10.1002/ijc.2910170107.
We have labelled the exposed surface glycoproteins of human blood T- and B-lymphocytes and cells from patients with chronic lymphocytic leukemia by the galactose oxidase-tritiated sodium borohydride method. The labelled glycoproteins were separated by polyacrylamide slab gel electrophoresis and visualized by autoradiography. The T- and B-lymphocytes show different and characteristic surface glycoprotein patterns. The surface glycoprotein patterns of the leukemic cells differ from those of normal, non-malignant lymphocytes. A relationship between the altered surface glycoprotein pattern of leukemic cells and the expression of leukemia-associated antigens is discussed.
我们采用半乳糖氧化酶-氚化硼氢化钠法对人血T淋巴细胞、B淋巴细胞以及慢性淋巴细胞白血病患者的细胞表面暴露的糖蛋白进行了标记。标记后的糖蛋白通过聚丙烯酰胺平板凝胶电泳进行分离，并用放射自显影法进行显影。T淋巴细胞和B淋巴细胞呈现出不同的特征性表面糖蛋白图谱。白血病细胞的表面糖蛋白图谱与正常非恶性淋巴细胞的图谱不同。本文讨论了白血病细胞表面糖蛋白图谱改变与白血病相关抗原表达之间的关系。
