Huguet E, Esponda P
Centro de Investigaciones Biológicas CSIC, Madrid, Spain.
Mol Reprod Dev. 2000 Jun;56(2 Suppl):243-7. doi: 10.1002/(SICI)1098-2795(200006)56:2+<243::AID-MRD6>3.0.CO;2-C.
Mouse vas deferens were injected with a plasmid DNA encoding the GFP (Green Fluorescent Protein). The night after injection males were mated with normal oestrus females, and the offspring were analyzed. From 53 newborns, 4 were found positive by PCR for the GFP gene. In these positive animals, some tissues showed expression for GFP as evidenced by a strong green cytoplasmic fluorescence. GFP expression was particularly patent in the liver (hepatocytes), kidney (renal corpuscle and tubules), abdominal wall, and lung. These preliminary results indicate the possibility to use this method as a simple alternative procedure to create transgenic animals, and it could be especially helpful in species in which the microinjection procedure is not feasible.
将编码绿色荧光蛋白（GFP）的质粒DNA注入小鼠输精管。注射后的当晚，雄性小鼠与处于正常发情期的雌性小鼠交配，并对后代进行分析。在53只新生小鼠中，通过PCR检测发现有4只小鼠的GFP基因呈阳性。在这些阳性动物中，一些组织呈现出GFP表达，表现为强烈的绿色细胞质荧光。GFP表达在肝脏（肝细胞）、肾脏（肾小体和肾小管）、腹壁和肺中尤为明显。这些初步结果表明，有可能将该方法作为创建转基因动物的一种简单替代方法，并且对于那些显微注射方法不可行的物种可能特别有用。
