Gottfried M, Orrego C, Keynan A, Halvorson H O
J Bacteriol. 1979 May;138(2):314-9. doi: 10.1128/jb.138.2.314-319.1979.
Spores of a Bacillus subtilis mutant temperature sensitive in deoxyribonucleic acid (DNA) replication proceeded through outgrowth at the nonpermissive temperature to the same extent as the wild-type parent spores. In contrast, the DNA synthesis inhibitor novobiocin completely prevented spore outgrowth while displaying a marginal effect on logarithmic growth during one generation time. Inhibition of outgrowth by novobiocin occurred in the absence of DNA replication, as demonstrated in an experiment with spores of the temperature-sensitive DNA synthesis mutant at the restrictive temperature. Novobiocin inhibited the initial rate of ribonucleic acid synthesis to the same extent in germinated spores and in exponentially growing cells. A novobiocin-resistant mutant underwent normal outgrowth in the presence of novobiocin. Therefore, novobiocin inhibition was independent of its effect on chromosome replication per se.
一种在脱氧核糖核酸（DNA）复制中对温度敏感的枯草芽孢杆菌突变体的孢子，在非允许温度下进行萌发的程度与野生型亲本孢子相同。相比之下，DNA合成抑制剂新生霉素完全阻止了孢子的萌发，而在一个世代时间内对对数生长仅表现出轻微影响。如在对温度敏感的DNA合成突变体的孢子在限制温度下进行的实验所示，新生霉素在没有DNA复制的情况下抑制了孢子的萌发。新生霉素在萌发的孢子和指数生长的细胞中对核糖核酸合成初始速率的抑制程度相同。一种对新生霉素耐药的突变体在有新生霉素存在的情况下能正常萌发。因此，新生霉素的抑制作用与其对染色体复制本身的影响无关。
