蜡样芽孢杆菌T生长过程中脱氧核糖核酸合成的性质及其与蛋白质合成的关系
Nature of deoxyribonucleic acid synthesis and its relationship to protein synthesis during outgrowth of Bacillus cereus T.
作者信息
Rana R S, Halvorson H O
出版信息
J Bacteriol. 1972 Feb;109(2):606-15. doi: 10.1128/jb.109.2.606-615.1972.
Abstract
Deoxyribonucleic acid (DNA) synthesis during early outgrowth of spores of Bacillus cereus T (thy(-)) has been examined. (14)C-thymidine incorporated begins 2 to 5 min after germination and continues at a slow rate up to 30 min, after which the rate of (14)C-thymidine incorporation increases considerably. Early DNA synthesis up to 30 min after germination is dependent upon simultaneous protein synthesis. The examination of the stability of proteins synthesized soon after germination shows that they are susceptible to intracellular degradation. The evidence provided here indicates that protein degradation is the cause of observed dependence of DNA synthesis on simultaneous protein synthesis. The DNA synthesis occurring soon after germination is primarily a repair type synthesis which is followed by the onset of normal replication approximately 30 min after germination.
摘要
已对蜡样芽孢杆菌T（thy(-)）孢子早期生长过程中的脱氧核糖核酸（DNA）合成进行了研究。掺入的（14）C-胸腺嘧啶核苷在萌发后2至5分钟开始，并以缓慢速率持续至30分钟，此后（14）C-胸腺嘧啶核苷掺入速率显著增加。萌发后30分钟内的早期DNA合成依赖于同时进行的蛋白质合成。对萌发后不久合成的蛋白质稳定性的检测表明，它们易受细胞内降解的影响。此处提供的证据表明，蛋白质降解是观察到的DNA合成对同时进行的蛋白质合成有依赖性的原因。萌发后不久发生的DNA合成主要是修复型合成，随后在萌发后约30分钟开始正常复制。
相似文献
1
Nature of deoxyribonucleic acid synthesis and its relationship to protein synthesis during outgrowth of Bacillus cereus T.蜡样芽孢杆菌T生长过程中脱氧核糖核酸合成的性质及其与蛋白质合成的关系
J Bacteriol. 1972 Feb;109(2):606-15. doi: 10.1128/jb.109.2.606-615.1972.
2
Method for restricting incorporation of radioactivity from 3 H-thymidine into deoxyribonucleic acid only during outgrowth of spores of Bacillus cereus T.仅在蜡样芽孢杆菌T型孢子生长期间限制³H-胸腺嘧啶核苷的放射性掺入脱氧核糖核酸的方法
J Bacteriol. 1972 Feb;109(2):599-605. doi: 10.1128/jb.109.2.599-605.1972.
3
Timing of enzyme synthesis during outgrowth of spores of Bacillus cereus. II. Relationship between ordered enzyme synthesis and deoxyribonucleic acid replication.蜡状芽孢杆菌芽孢萌发过程中酶合成的时间。II. 有序酶合成与脱氧核糖核酸复制之间的关系。
J Bacteriol. 1968 Feb;95(2):479-89. doi: 10.1128/jb.95.2.479-489.1968.
4
Relationship between protein and ribonucleic acid synthesis during outgrowth of spores of Bacillus cereus.蜡样芽孢杆菌孢子萌发过程中蛋白质与核糖核酸合成之间的关系。
J Bacteriol. 1968 Aug;96(2):492-500. doi: 10.1128/jb.96.2.492-500.1968.
5
Outgrowth of Bacillus cereus spores harboring bacteriophage CP-51 DNA. I. Initiation of bacteriophage development.携带噬菌体CP - 51 DNA的蜡样芽孢杆菌孢子的生长。I. 噬菌体发育的起始
J Virol. 1973 May;11(5):648-54. doi: 10.1128/JVI.11.5.648-654.1973.
6
Microcycle sporogenesis of Bacillus cereus in a chemically defined medium.蜡样芽孢杆菌在化学限定培养基中的微周期孢子形成
J Bacteriol. 1968 Feb;95(2):355-9. doi: 10.1128/jb.95.2.355-359.1968.
7
Phase transitions in ribonucleic acid synthesis during germination of Bacillus subtilis spores.枯草芽孢杆菌孢子萌发过程中核糖核酸合成的相变
Proc Natl Acad Sci U S A. 1968 Jan;59(1):153-60. doi: 10.1073/pnas.59.1.153.
8
Spores of microorganisms. XXVI. Synthetic activities of germinating spores of Bacillus cereus prevented from outgrowth.微生物孢子。二十六。阻止生长的蜡样芽孢杆菌萌发孢子的合成活性。
Folia Microbiol (Praha). 1975;20(3):195-205. doi: 10.1007/BF02876779.
9
Lipid metabolism during bacterial growth, sporulation, and germination: kinetics of fatty acid and macromolecular synthesis during spore germination and outgrowth of Bacillus thuringiensis.细菌生长、芽孢形成和萌发过程中的脂质代谢：苏云金芽孢杆菌芽孢萌发和生长过程中脂肪酸及大分子合成的动力学
J Bacteriol. 1975 Jan;121(1):227-33. doi: 10.1128/jb.121.1.227-233.1975.
10
Repair of radiation damage to deoxyribonucleic acid in germinating spores of Bacillus subtilis.枯草芽孢杆菌萌发孢子中脱氧核糖核酸辐射损伤的修复
J Bacteriol. 1971 Jun;106(3):925-30. doi: 10.1128/jb.106.3.925-930.1971.
引用本文的文献
1
Analysis of temporal gene expression during Bacillus subtilis spore germination and outgrowth.枯草芽孢杆菌孢子萌发和生长过程中的时间基因表达分析。
J Bacteriol. 2007 May;189(9):3624-34. doi: 10.1128/JB.01736-06. Epub 2007 Feb 23.
2
Percent charging of transfer ribonucleic acid and levels of ppGpp and pppGpp in dormant and germinated spores of Bacillus megaterium.巨大芽孢杆菌休眠和萌发孢子中转录核糖核酸的充电百分比以及ppGpp和pppGpp的水平
J Bacteriol. 1974 Jun;118(3):1067-74. doi: 10.1128/jb.118.3.1067-1074.1974.
3
Deoxyribonucleic acid synthesis and deoxynucleotide metabolism during bacterial spore germination.细菌孢子萌发过程中的脱氧核糖核酸合成与脱氧核苷酸代谢
J Bacteriol. 1973 Jun;114(3):1099-107. doi: 10.1128/jb.114.3.1099-1107.1973.
4
The preferential inhibition of Bacillus subtilis spore outgrowth by chloroquine.氯喹对枯草芽孢杆菌孢子萌发的优先抑制作用。
Arch Microbiol. 1989;152(3):251-7. doi: 10.1007/BF00409659.
5
Lipid metabolism during bacterial growth, sporulation, and germination: kinetics of fatty acid and macromolecular synthesis during spore germination and outgrowth of Bacillus thuringiensis.细菌生长、芽孢形成和萌发过程中的脂质代谢：苏云金芽孢杆菌芽孢萌发和生长过程中脂肪酸及大分子合成的动力学
J Bacteriol. 1975 Jan;121(1):227-33. doi: 10.1128/jb.121.1.227-233.1975.
6
Macromolecular synthesis accompanying the transition from spores to vegetative forms of Streptomyces granaticolor.与石榴色链霉菌从孢子转变为营养体形式相关的大分子合成。
Folia Microbiol (Praha). 1977;22(4):252-61. doi: 10.1007/BF02877654.
7
Nucleic acid synthesis and ribonucleic acid polymerase specificity in germinating and outgrowing spores of Bacillus subtilis.枯草芽孢杆菌萌发和生长中的孢子中的核酸合成与核糖核酸聚合酶特异性
J Bacteriol. 1975 Oct;124(1):190-200. doi: 10.1128/jb.124.1.190-200.1975.
8
Microbial growth and production of antibiotics.微生物生长与抗生素生产
Folia Microbiol (Praha). 1978;23(4):309-28. doi: 10.1007/BF02876686.
9
Specific inhibition of outgrowth of Bacillus subtilis spores by novobiocin.新生霉素对枯草芽孢杆菌孢子生长的特异性抑制作用。
J Bacteriol. 1979 May;138(2):314-9. doi: 10.1128/jb.138.2.314-319.1979.
10
Independence of Bacillus subtilis spore outgrowth from DNA synthesis.枯草芽孢杆菌孢子萌发与DNA合成的独立性。
J Bacteriol. 1978 Oct;136(1):111-6. doi: 10.1128/jb.136.1.111-116.1978.
本文引用的文献
1
Thymine Photoproducts but not Thymine Dimers Found in Ultraviolet-Irradiated Bacterial Spores.在紫外辐射的细菌孢子中发现胸腺嘧啶光产物而不是胸腺嘧啶二聚体。
Science. 1965 Jul 16;149(3681):308-10. doi: 10.1126/science.149.3681.308.
2
SEQUENTIAL REPLICATION OF THE BACILLUS SUBTILIS CHROMOSOME. 3. REGULATION OF INITIATION.枯草芽孢杆菌染色体的连续复制。3. 起始调控。
Proc Natl Acad Sci U S A. 1964 Oct;52(4):973-80. doi: 10.1073/pnas.52.4.973.
3
SEQUENTIAL REPLICATION OF DNA IN SYNCHRONOUSLY GERMINATING BACILLUS SUBTILIS SPORES.枯草芽孢杆菌同步萌发孢子中DNA的连续复制
Biochem Biophys Res Commun. 1963 Sep 10;13:67-70. doi: 10.1016/0006-291x(63)90164-5.
4
Sequential replication of Bacillus subtilis chromosome. I. Comparison of marker frequencies in exponential and stationary growth phases.枯草芽孢杆菌染色体的顺序复制。I. 指数生长期和稳定生长期标记频率的比较。
Proc Natl Acad Sci U S A. 1963 Apr;49(4):559-66. doi: 10.1073/pnas.49.4.559.
5
[Ribonucleic acid from Bacillus subtilis spores].[来自枯草芽孢杆菌孢子的核糖核酸]
Biochim Biophys Acta. 1963 Jul 30;72:497-500. doi: 10.1016/0006-3002(63)90272-5.
6
Pattern of synthesis of deoxyribonucleic acid in Bacillus cereus growing synchronously out of spores.蜡样芽孢杆菌从孢子中同步生长时脱氧核糖核酸的合成模式
Nature. 1959 Feb 7;183(4658):372-3. doi: 10.1038/183372a0.
7
Studies on spore germination: its independence from alanine racemase activity.孢子萌发研究：其与丙氨酸消旋酶活性无关。
J Bacteriol. 1954 Oct;68(4):393-9. doi: 10.1128/jb.68.4.393-399.1954.
8
Regulation of nucleic acid synthesis in Lactobacillus acidophilus R-26.嗜酸乳杆菌R-26中核酸合成的调控
Biochim Biophys Acta. 1966 Jun 22;119(3):526-39. doi: 10.1016/0005-2787(66)90129-8.
9
Increase of thymidine, thymidylate and deoxycytidine kinase activites during germination of bacterial spores.细菌芽孢萌发过程中胸苷、胸苷酸和脱氧胞苷激酶活性的增加。
Biochim Biophys Acta. 1971 Jan 1;228(1):26-37. doi: 10.1016/0005-2787(71)90543-0.
10
Germination-induced repair of single-strand breaks of DNA in irradiated Bacillus subtilis spores.发芽诱导辐照枯草芽孢杆菌孢子中DNA单链断裂的修复。
Biochem Biophys Res Commun. 1969 Sep 24;37(1):66-71. doi: 10.1016/0006-291x(69)90881-x.
Zotero 插件