脑膜炎奈瑟菌中嘧啶碱基和核苷的代谢
Metabolism of pyrimidine bases and nucleosides in Neisseria meningitidis.
作者信息
Jyssum S, Jyssum K
出版信息
J Bacteriol. 1979 May;138(2):320-3. doi: 10.1128/jb.138.2.320-323.1979.
In Neisseria meningitidis, uridine, deoxyuridine, cytosine, cytidine, or deoxycytidine could not be used by uracil-requiring mutants as pyrimidine sources. Consistent with these findings, only 5-fluorouracil of the different fluoropyrimidine bases and nucleosides showed any inhibitory effect on the growth of four prototrophic strains of N. meningitidis. Likewise, only radioactive uracil was readily incorporated into nucleic acids, whereas uptake of radioactive uridine, cytosine, or cytidine could not be demonstrated. Uracil was converted to uridine 5'-monophosphate by uracil phosphoribosyltransferase, whereas enzyme activities for conversion of cytosine or any of the nucleosides were not detectable in meningococcal extracts.
在脑膜炎奈瑟菌中，尿嘧啶、脱氧尿嘧啶、胞嘧啶、胞苷或脱氧胞苷不能被需要尿嘧啶的突变体用作嘧啶来源。与这些发现一致的是，在不同的氟嘧啶碱基和核苷中，只有5-氟尿嘧啶对脑膜炎奈瑟菌的四株原养型菌株的生长有抑制作用。同样，只有放射性尿嘧啶能很容易地掺入核酸中，而放射性尿苷、胞嘧啶或胞苷的摄取则无法检测到。尿嘧啶通过尿嘧啶磷酸核糖基转移酶转化为5'-单磷酸尿苷，而在脑膜炎球菌提取物中未检测到胞嘧啶或任何核苷转化的酶活性。
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