Pikuta E, Lysenko A, Suzina N, Osipov G, Kuznetsov B, Tourova T, Akimenko V, Laurinavichius K
Int J Syst Evol Microbiol. 2000 Jan;50 Pt 1:25-33. doi: 10.1099/00207713-50-1-25.
A new moderately thermophilic, alkaliphilic, sulfate-reducing, chemolithoheterotrophic bacterium, strain S1T, was isolated from a mixed cow/pig manure with neutral pH. The bacterium is an obligately anaerobic, non-motile, Gram-positive, spore-forming curved rod growing within a pH range of 8.0-9.15 (optimal growth at pH 8.6-8.7) and temperature range of 30-58 degrees C (optimal growth at 50-55 degrees C). The optimum NaCl concentration for growth is 0.1%. Strain S1T is an obligately carbonate-dependent alkaliphile. The G+C content of the DNA is 40.9 mol%. A limited number of compounds are utilized as electron donors, including H2+acetate, formate, ethanol, lactate and pyruvate. Sulfate, sulfite and thiosulfate, but not sulfur or nitrate, can be used as electron acceptors. Strain S1T is able to utilize acetate or yeast extract as sources of carbon. Analysis of the 16S rDNA sequence allowed strain S1T (= DSM 12257T) to be classified as a representative of a new species of the genus Desulfotomaculum, Desulfotomaculum alkaliphilum sp. nov.
从pH值为中性的奶牛/猪混合粪便中分离出一种新的嗜中温、嗜碱、硫酸盐还原、化能无机异养细菌，菌株S1T。该细菌为专性厌氧、不运动、革兰氏阳性、产芽孢弯曲杆菌，生长pH范围为8.0 - 9.15（pH 8.6 - 8.7时生长最佳），温度范围为30 - 58℃（50 - 55℃时生长最佳）。生长的最佳NaCl浓度为0.1%。菌株S1T是专性依赖碳酸盐的嗜碱菌。DNA的G+C含量为40.9 mol%。有限数量的化合物被用作电子供体，包括H₂+乙酸盐、甲酸盐、乙醇、乳酸盐和丙酮酸盐。硫酸盐、亚硫酸盐和硫代硫酸盐可用作电子受体，但硫或硝酸盐不行。菌株S1T能够利用乙酸盐或酵母提取物作为碳源。对16S rDNA序列的分析使菌株S1T（= DSM 12257T）被归类为脱硫肠状菌属一个新物种——嗜碱脱硫肠状菌的代表种Desulfotomaculum alkaliphilum sp. nov.
