Bonilla Salinas Monica, Fardeau Marie-Laure, Thomas Pierre, Cayol Jean-Luc, Patel Bharat K C, Ollivier Bernard
IRD, UR 101 Extrêmophiles, IFR-BAIM, Universités de Provence et de la Méditerranée, ESIL, Marseille, France.
School of Biomolecular and Biomedical Sciences, Griffith University, Brisbane, Australia.
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2169-2173. doi: 10.1099/ijs.0.02926-0.
A novel Gram-positive, anaerobic and moderately thermophilic bacterium, strain 50-1 BON(T), was isolated from an Australian terrestrial oil reservoir. Cells were spore-forming straight rods, motile by peritrichous flagella. The optimum growth conditions were 50 degrees C, pH 7.5 and 0.1 % NaCl. Strain 50-1 BON(T) fermented arabinose, cellobiose, fructose, galactose, glucose, mannose, sucrose, xylose and yeast extract. Glucose was fermented mainly into lactate, formate, hydrogen and CO(2). The major end product of pyruvate fermentation was acetate together with H(2) and CO(2). Thiosulfate, sulfate, elemental sulfur and nitrate were not used as terminal electron acceptors. The DNA G+C content was 55.5 mol%. The closest phylogenetic relative of strain 50-1 BON(T) was Thermoanaerobacterium thermosulfurigenes (16S rRNA gene sequence similarity of 85.7 %). As strain 50-1 BON(T) was physiologically and phylogenetically different from members of the order 'Thermoanaerobacteriales', it is proposed that strain 50-1 BON(T) (=DSM 15567(T)=CIP 107919(T)) be classified as the type strain of a novel species of a new genus, Mahella australiensis gen. nov., sp. nov.
从澳大利亚陆地油藏中分离出一株新型革兰氏阳性、厌氧且嗜热适度的细菌，菌株50-1 BON(T)。细胞为产芽孢直杆状，通过周生鞭毛运动。最佳生长条件为50℃、pH 7.5和0.1% NaCl。菌株50-1 BON(T)可发酵阿拉伯糖、纤维二糖、果糖、半乳糖、葡萄糖、甘露糖、蔗糖、木糖和酵母提取物。葡萄糖主要发酵生成乳酸、甲酸、氢气和CO₂。丙酮酸发酵的主要终产物是乙酸以及H₂和CO₂。硫代硫酸盐、硫酸盐、元素硫和硝酸盐不用作末端电子受体。DNA的G+C含量为55.5 mol%。菌株50-1 BON(T)最接近的系统发育亲缘种是嗜热产硫化物嗜热厌氧杆菌（16S rRNA基因序列相似性为85.7%）。由于菌株50-1 BON(T)在生理和系统发育上与“嗜热厌氧杆菌目”的成员不同，因此建议将菌株50-1 BON(T)（=DSM 15567(T)=CIP 107919(T)）分类为一个新属——澳大利亚马氏菌属（Mahella australiensis gen. nov.）的一个新种的模式菌株。
