Kaksonen Anna H, Spring Stefan, Schumann Peter, Kroppenstedt Reiner M, Puhakka Jaakko A
Institute of Environmental Engineering and Biotechnology, Tampere University of Technology, Tampere, Finland.
DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
Int J Syst Evol Microbiol. 2006 Nov;56(Pt 11):2603-2608. doi: 10.1099/ijs.0.64439-0.
A thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from an underground mine in a geothermally active area in Japan. Cells of this strain, designated RL50JIIIT, were rod-shaped and motile. The temperature range for growth was 50-72 degrees C (optimum growth at 61-66 degrees C) and the pH range was 6.4-7.8 (optimum at pH 7.2-7.4). Strain RL50JIIIT tolerated up to 1.5% NaCl, but optimum growth occurred in the presence of 0-1% NaCl. Electron acceptors utilized were sulfate, sulfite, thiosulfate and elemental sulfur. Electron donors utilized were H2 in the presence of CO2, alanine, various carboxylic acids and alcohols. Fermentative growth occurred on lactate and pyruvate. The cell wall contained mesodiaminopimelic acid and the major respiratory isoprenoid quinone was menaquinone 7 (MK-7). Major whole-cell fatty acids were iso-C15:0, iso-C17:0 DMA (dimethyl acetal), iso-C15:0 DMA and iso-C17:0. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed 98.7% similarity with Desulfotomaculum solfataricum DSM 14956T. However, DNA-DNA hybridization experiments with Desulfotomaculum kuznetsovii, Desulfotomaculum luciae and D. solfataricum and the G+C content of the DNA (54.4 mol%) allowed the differentiation of strain RL50JIIIT from the recognized species of the genus Desulfotomaculum. Strain RL50JIIIT therefore represents a novel species, for which the name Desulfotomaculum thermosubterraneum sp. nov. is proposed. The type strain is RL50JIIIT (=DSM 16057T=JCM 13837T).
从日本一个地热活跃地区的地下矿井中分离出一株嗜热、革兰氏阳性、形成芽孢、硫酸盐还原细菌。该菌株命名为RL50JIIIT，细胞呈杆状且具运动性。生长温度范围为50 - 72℃（最适生长温度为61 - 66℃），pH范围为6.4 - 7.8（最适pH为7.2 - 7.4）。RL50JIIIT菌株可耐受高达1.5%的NaCl，但在0 - 1% NaCl存在时生长最佳。利用的电子受体为硫酸盐、亚硫酸盐、硫代硫酸盐和元素硫。利用的电子供体为CO2存在下的H2、丙氨酸、各种羧酸和醇。在乳酸盐和丙酮酸盐上可进行发酵生长。细胞壁含有内消旋二氨基庚二酸，主要呼吸类异戊二烯醌为甲基萘醌7（MK - 7）。主要的全细胞脂肪酸为异C15:0、异C17:0 DMA（二甲基缩醛）、异C15:0 DMA和异C17:0。基于16S rRNA基因序列比较的系统发育分析显示，与嗜热栖热脱硫肠状菌DSM 14956T的相似性为98.7%。然而，与库兹涅佐夫脱硫肠状菌、卢西亚脱硫肠状菌和嗜热栖热脱硫肠状菌进行的DNA - DNA杂交实验以及DNA的G + C含量（54.4 mol%）使得RL50JIIIT菌株能够与脱硫肠状菌属已确认的物种区分开来。因此，RL50JIIIT菌株代表一个新物种，提议将其命名为嗜热地下脱硫肠状菌（Desulfotomaculum thermosubterraneum sp. nov.）。模式菌株为RL50JIIIT（=DSM 16057T=JCM 13837T）。
