Goldman J M, Th'ng K H, Catovsky D, Galton D A
Blood. 1976 Mar;47(3):381-8.
The production of colony-stimulating factor (CSF) by the peripheral blood cells of untreated patients with acute myeloid leukemia (AML) was measured in the agar culture system using normal human bone marrow as the source of colony-forming units (CFUc). CSF production was found to be variable and was related to the morphologic subtype of AML--cells from patients with monocytic leukemia produced normal or large quantities of CSF, while (with one exception) those from patients with myeloblastic leukemia produced little or no CSF. There was a general relationship between CSF production and serum lysozyme levels. Attempts to demonstrate a consistent inhibitory effect exerted by leukemic peripheral blood cells on normal leukopoiesis in vitro were negative. Results instead suggested that the addition to the feeder layer of cells from patients with monocytic leukemia could raise CSF levels above those obtained with normal peripheral blood leukocytes alone, possibly by recruiting additional CFUc from normal marrow.
在琼脂培养系统中，以正常人骨髓作为集落形成单位（CFUc）的来源，检测了未经治疗的急性髓性白血病（AML）患者外周血细胞产生集落刺激因子（CSF）的情况。发现CSF的产生存在差异，且与AML的形态学亚型有关——单核细胞白血病患者的细胞产生正常量或大量的CSF，而（有一个例外）髓母细胞白血病患者的细胞产生很少或不产生CSF。CSF产生与血清溶菌酶水平之间存在普遍关系。试图证明白血病外周血细胞在体外对正常白细胞生成具有一致抑制作用的尝试均为阴性。相反，结果表明，向饲养层中添加单核细胞白血病患者的细胞可能会使CSF水平高于仅使用正常外周血白细胞时的水平，这可能是通过从正常骨髓中募集额外的CFUc实现的。
