Thulesen J, Nexø E, Poulsen S S
Institute of Medical Anatomy, Depart. B, The Panum Institute, University of Copenhagen, Blegdamsvej 3, 2200 N, Copenhagen, Denmark.
Regul Pept. 2000 Jun 30;90(1-3):69-76. doi: 10.1016/s0167-0115(00)00111-7.
This study investigates the renal and urinary levels of epidermal growth factor (EGF) in rats under long-term treatment with alpha- or beta-adrenergic agonists. Urine samples were obtained on days 7, 14 and 21, and renal tissue samples on day 21. EGF was quantified by ELISA and tissue sections were used for immunohistochemistry and in situ hybridization. Fractional kidney weight was increased in the alpha-adrenergic agonist-treated group by 35% when compared with controls. Histological examination of the kidney revealed well-defined wedge-shaped areas of tubular dilatations and luminal amorphous material in the distal tubules. Concomitantly, reduced levels of EGF and EGF mRNA were observed, and also the urinary levels of EGF were reduced. Together, these observations indicate alpha-adrenergic treatment to affect the distal tubules. Treatment with the beta-adrenergic agonist did not change fractional kidney weight, but initially the urinary excretion of EGF was reduced. The data add further evidence to the suggestion that activity of the sympathetic nervous system influences renal homeostasis of EGF, either directly or indirectly through renal histopathological changes.
本研究调查了长期用α或β肾上腺素能激动剂治疗的大鼠肾脏和尿液中表皮生长因子（EGF）的水平。在第7、14和21天采集尿液样本，在第21天采集肾脏组织样本。通过酶联免疫吸附测定法对EGF进行定量，并将组织切片用于免疫组织化学和原位杂交。与对照组相比，α肾上腺素能激动剂治疗组的肾脏相对重量增加了35%。肾脏组织学检查显示，在远端肾小管中有明确界定的楔形肾小管扩张区域和管腔内无定形物质。同时，观察到EGF和EGF mRNA水平降低，尿液中EGF水平也降低。这些观察结果共同表明，α肾上腺素能治疗会影响远端肾小管。用β肾上腺素能激动剂治疗并未改变肾脏相对重量，但最初EGF的尿排泄量减少。这些数据进一步证明了交感神经系统的活动直接或通过肾脏组织病理学变化间接影响EGF的肾脏内稳态这一观点。
