Segel G B, Gordon B R, Lichtman M A, Hollander M M, Klemperer M R
J Cell Physiol. 1976 Mar;87(3):337-43. doi: 10.1002/jcp.1040870309.
We have found that PHA produces an alteration in the lymphocyte membrane which allows 86Rb+ or 42K+ in prelabeled lymphocytes to exchange for cations present in washing solutions. These observations suggested that PHA might induce an increase in the exodus of intracellular potassium during incubation in physiologic media. We, therefore, examined 86Rb+ and 42K+ efflux from rat and human lymphocytes during incubation in tissue culture medium. The rate constant for efflux, Ke, was significantly increased by PHA. 86Rb+ efflux was increased by 27% in rat thymic lymphocytes and by 78% in human blood lymphocytes following PHA treatment.
我们发现，PHA会使淋巴细胞膜发生改变，从而使预先标记的淋巴细胞中的86Rb+或42K+能够与洗涤液中的阳离子进行交换。这些观察结果表明，PHA可能会在生理介质中孵育期间诱导细胞内钾外流增加。因此，我们检测了在组织培养基中孵育期间大鼠和人类淋巴细胞的86Rb+和42K+外流情况。PHA显著增加了外流速率常数Ke。经PHA处理后，大鼠胸腺淋巴细胞中的86Rb+外流增加了27%，人类血液淋巴细胞中的86Rb+外流增加了78%。
