小鼠 - 人异核体细胞表面抗原的扩散速率。II. 膜电位对侧向扩散的影响。
Diffusion rates of cell surface antigens of mouse-human heterokaryons. II. Effect of membrane potential on lateral diffusion.
作者信息
Edidin M, Wei T Y
出版信息
J Cell Biol. 1977 Nov;75(2 Pt 1):483-9. doi: 10.1083/jcb.75.2.483.
Abstract
The rate of appearance, in a population of mouse-human heterokaryons, of cells with intermixed mouse and human surface antigens may be used to estimate the rate of lateral diffusion of the antigens in a single cell. Most heterokaryons appear to restrict diffusion of their surface antigens. These restrictions are altered by exposing either heterokaryons or their parent cells to conditions that change cell surface membrane potential. Media containing unphysiological concentrations of potassium ion, drugs, affecting the Na+,K+ ATPase, or a channel-forming antibiotic, gramicidin, all affect lateral mobility of cell surface antigens in a manner consistent with a common effect on membrane potential.
摘要
在小鼠 - 人异核体细胞群体中，具有混合的小鼠和人表面抗原的细胞出现的速率，可用于估计单个细胞中抗原的侧向扩散速率。大多数异核体似乎会限制其表面抗原的扩散。通过将异核体或其亲本细胞暴露于改变细胞表面膜电位的条件下，这些限制会发生改变。含有非生理浓度钾离子的培养基、影响Na +，K + ATP酶的药物或形成通道的抗生素短杆菌肽，都会以与对膜电位的共同影响一致的方式影响细胞表面抗原的侧向流动性。
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