Fujimoto S, Greene M I, Sehon A H
J Immunol. 1976 Mar;116(3):791-9.
A/Jax mice were rendered immune to the syngeneic and transplantable methylcholanthrene-induced Sarcoma 1509a by the surgical removal of the tumor 7 days after implantation; subsequent injection i.v. transfer of 10(7) to 10(8) washed thymus or spleen cells of tumor-bearing animals (TBA) to immune animals significantly inhibited the rejection of the tumor; this suppressive effect was entirely abolished by the treatment of these lymphocytes with anti-theta serum or anti-thymocyte serum (ATS) and complement before adoptive transfer. On the other hand, an equal number of thymus or spleen cells of normal animals or of animals bearing an unrelated tumor had no suppressive effect. Treatment of normal syngeneic animals with ATS after tumor cell inoculation or splenectomy of TBA resulted in the suppression of the tumor growth. The serum of TBA had no effect on tumor growth in immune syngeneic mice. Together these results suggest that TBA possess immunosuppressor T cells regulating negatively their immune response to the tumor.
通过在植入后7天手术切除肿瘤，使A/Jax小鼠对同基因且可移植的甲基胆蒽诱导的肉瘤1509a产生免疫；随后经静脉注射将10⁷至10⁸个来自荷瘤动物（TBA）的洗涤过的胸腺或脾细胞转移至免疫动物体内，可显著抑制肿瘤的排斥反应；在用抗θ血清或抗胸腺细胞血清（ATS）及补体处理这些淋巴细胞后进行过继转移，这种抑制作用完全消失。另一方面，正常动物或携带无关肿瘤动物的等量胸腺或脾细胞则无抑制作用。在肿瘤细胞接种后用ATS处理同基因正常动物或对TBA进行脾切除，可导致肿瘤生长受到抑制。TBA的血清对免疫同基因小鼠的肿瘤生长无影响。这些结果共同表明，TBA拥有免疫抑制性T细胞，可对其针对肿瘤的免疫反应进行负向调节。
