Imaya H, Kudo M, Matsuura H
Department of Neurosurgery, Toho University School of Medicine, Tokyo, Japan.
Int J Exp Pathol. 1993 Feb;74(1):73-7.
We have succeeded in inducing progressive splenomegaly by inoculating 9L-glioma cells into the back subcutaneous regions of Fischer rats. The progressive splenomegaly was histologically characterized by progressively increased extramedullary haematopoiesis. Immunohistochemically Leu 7 positive cells were present in the enlarged spleen, suggesting that the spleen possessed natural killer (NK) cells. However, the splenic NK cell activity obtained in a 4h-51Cr release assay at 5 weeks after inoculation was below 5%, suggesting that the cytotoxic function of the NK cells was depressed. Bone marrow morphologically appeared not to be suppressed. Although the exact cause and mechanism of the progressive splenomegaly with prominent extramedullary haematopoiesis and severe suppression of the splenic NK cell activity remains speculative at present, it could be a unique manifestation of a systemic host-reaction reflected by the 9L-glioma continuously growing in the subcutaneous regions. This experiment could be a useful approach to the study of splenic and other reticuloendothelial roles in host-immunity in the tumour bearing state.
我们通过将9L胶质瘤细胞接种到Fischer大鼠背部皮下区域成功诱导出进行性脾肿大。进行性脾肿大在组织学上的特征是髓外造血逐渐增加。免疫组织化学显示，肿大的脾脏中存在Leu 7阳性细胞，提示脾脏拥有自然杀伤（NK）细胞。然而，接种后5周在4小时51铬释放试验中测得的脾脏NK细胞活性低于5%，提示NK细胞的细胞毒性功能受到抑制。骨髓形态学上似乎未受抑制。尽管目前尚不清楚伴有显著髓外造血和脾脏NK细胞活性严重抑制的进行性脾肿大的确切原因和机制，但它可能是皮下区域持续生长的9L胶质瘤所反映的全身宿主反应的一种独特表现。该实验可能是研究荷瘤状态下脾脏及其他网状内皮系统在宿主免疫中作用的一种有用方法。
