Böhm H, Anthony P, Davey M R, Briarty L G, Power J B, Lowe K C, Benes E, Gröschl M
Plant Science Division, School of Biological Sciences, University of Nottingham, UK.
Ultrasonics. 2000 Mar;38(1-8):629-32. doi: 10.1016/s0041-624x(99)00166-3.
Exposure of Petunia hybrida cell suspensions to ultrasound at a frequency of 2.43 MHz in a standing wave field at an energy density of 70 Jm-3 (pressure amplitude of 0.78 MPa) decreased their mean viability to 35% after 20 min of sonication. A comparison of propagating wave and standing wave treatments at equal frequency (2.15 MHz) and energy density (8.5 Jm-3) showed, in the first case, a rapid decline in mean viability of cells (to 30% after 10 min of sonication) and, in the second case, a retaining of the initial viability (95%), respectively. Cells sonicated 4 days after subculture were more sensitive than cells sonicated 2 or 6 days after transfer to new culture medium. It was concluded that cellular viability depends primarily on the acoustic energy density, the exposure time, and the mechanical properties of the cells determined by age. As a consequence of the trapping of cells in the anti-node planes of the standing wave, propagating wave fields reduced cellular viability compared with standing wave fields at equal energy density.
在驻波场中，将矮牵牛细胞悬浮液暴露于频率为2.43 MHz、能量密度为70 Jm-3（压力幅值为0.78 MPa）的超声波下，超声处理20分钟后，其平均活力降至35%。在相同频率（2.15 MHz）和能量密度（8.5 Jm-3）下，对行波处理和驻波处理进行比较，结果显示，在第一种情况下，细胞平均活力迅速下降（超声处理10分钟后降至30%），而在第二种情况下，细胞初始活力得以保持（95%）。继代培养4天后进行超声处理的细胞比转移至新培养基2天或6天后进行超声处理的细胞更敏感。得出的结论是，细胞活力主要取决于声能密度、暴露时间以及由细胞年龄决定的机械性能。由于细胞被困在驻波的波腹平面中，与相同能量密度的驻波场相比，行波场降低了细胞活力。
