Khedr Mogibelrahman M S, Messaoudi Walid, Jonnalagadda Umesh S, Abdelmotelb Ahmed M, Glynne-Jones Peter, Hill Martyn, Khakoo Salim I, Abu Hilal Mohammed
Clinical and Experimental Sciences Academic Unit, Faculty of Medicine, University of Southampton, Southampton SO16 6YD, United Kingdom
Faculty of Medicine, Suez Canal University, Ismailia 41111, Egypt
Biomicrofluidics. 2019 Feb 12;13(1):014112. doi: 10.1063/1.5082603. eCollection 2019 Jan.
Ultrasonic standing wave systems have previously been used for the generation of 3D constructs for a range of cell types. In the present study, we cultured cells from the human hepatoma Huh7 cell line in a Bulk Acoustic Wave field and studied their viability, their functions, and their response to the anti-cancer drug, 5 Fluorouracil (5FU). We found that cells grown in the acoustofluidic bioreactor (AFB) expressed no reduction in viability up to 6 h of exposure compared to those cultured in a conventional 2D system. In addition, constructs created in the AFB and subsequently cultured outside of it had improved functionality including higher albumin and urea production than 2D or pellet cultures. The viability of Huh7 cells grown in the ultrasound field to 5FU anti-cancer drug was comparable to that of cells cultured in the 2D system, showing rapid diffusion into the aggregate core. We have shown that AFB formed 3D cell constructs have improved functionality over the conventional 2D monolayer and could be a promising model for anti-cancer drug testing.
超声驻波系统此前已用于为一系列细胞类型生成3D构建体。在本研究中，我们将人肝癌Huh7细胞系的细胞培养在体声波场中，并研究了它们的活力、功能以及它们对抗癌药物5-氟尿嘧啶（5FU）的反应。我们发现，与在传统二维系统中培养的细胞相比，在声流体生物反应器（AFB）中生长的细胞在长达6小时的暴露时间内活力没有降低。此外，在AFB中创建并随后在其外培养的构建体具有改善的功能，包括比二维或微球培养产生更高的白蛋白和尿素。在超声场中生长的Huh7细胞对5FU抗癌药物的活力与在二维系统中培养的细胞相当，显示出药物迅速扩散到聚集体核心。我们已经表明，AFB形成的3D细胞构建体比传统的二维单层具有更好的功能，并且可能是抗癌药物测试的一个有前景的模型。
