Radel S, McLoughlin A J, Gherardini L, Doblhoff-Dier O, Benes E
Department of Industrial Microbiology, University College Dublin, Ireland.
Ultrasonics. 2000 Mar;38(1-8):633-7. doi: 10.1016/s0041-624x(99)00211-5.
Recent studies have shown that there is no loss of cell viability when the cells are subjected to ultrasonic standing wave fields in acoustic cell retention systems. These systems are characterised by waves that spatially vary in pressure amplitude in the direction of sound propagation. In this work an anechoic 'one-dimensional' sonication chamber has been developed that produces propagating waves, which differ from standing waves in that the pressure amplitude remains constant as the wave travels in a medium with negligible attenuation. The viability of yeast cell suspensions as a function of treatment time was investigated during exposure to both standing and propagating wave fields with frequencies slightly above 2 MHz. The influence of 12% (vol/vol) of ethanol in water on the spatial arrangement of the cells in suspension was also studied. Changes in yeast cell morphology caused by the different types of suspension media and the ultrasonic treatment were examined by transmission electron microscopy (TEM). The agglomeration of yeast cells within the pressure nodal planes appears to minimise damaging effects due to ultrasonic fields.
最近的研究表明，在声学细胞滞留系统中，当细胞受到超声驻波场作用时，细胞活力不会丧失。这些系统的特点是，在声音传播方向上，压力振幅在空间上变化的波。在这项工作中，开发了一种消声的“一维”超声处理室，它产生传播波，与驻波不同的是，当波在衰减可忽略不计的介质中传播时，压力振幅保持恒定。在暴露于频率略高于2MHz的驻波场和传播波场期间，研究了酵母细胞悬液的活力与处理时间的函数关系。还研究了12%（体积/体积）的乙醇水溶液对悬浮液中细胞空间排列的影响。通过透射电子显微镜（TEM）检查了不同类型的悬浮介质和超声处理对酵母细胞形态的影响。酵母细胞在压力节点平面内的团聚似乎使超声场造成的损伤效应最小化。
