Thiel E, Rodt H, Huhn D, Thierfelder S
Blood. 1976 May;47(5):723-36.
B- and T-cell markers were studied in a patient with chronic lymphocytic leukemia and erythroderma. The absence of immunoglobulin, complement receptor, and Fc receptor, and the presence of sheep erythrocyte receptor and T-cell antigen on the membrane of the leukemic cells classified them as thymus derived. Using quantitative microphotometric immunoautoradiography, surface antigen densities were measured at the cellular level with the following results: (1) The density of T-antigenic sites was less on leukemic cells compared to normal T lymphocytes. (2) The T-antigen densities of leukemic lymphocytes varied less from cell to cell forming a homogeneous peak in histograms. (3) An Ig density of normal B lymphocytes was demonstrated on the residual T-antigen-negative cells. The results were qualitatively confirmed by direct immunofluorescence and electron microscopy with peroxidase-labeled antibodies. Furthermore, the surface antigens were quantitative microcomplement fixation test which revealed reduced binding of anti-T-cell antibodies and complement, and no antiglobulin fixation on the leukemic lymphocytes. Since lymphocytes with normal T-antigen concentration could not be found among the leukemic T lymphocytes, a lack of normal T cells was assumed. The findings that there was a decrease and altered distribution of surface markers on chronic lymphatic leukemia cells of the B- and T-cell type are discussed as further arguments referring to their clonal origin.
对一名慢性淋巴细胞白血病伴红皮病患者的B细胞和T细胞标志物进行了研究。白血病细胞膜上缺乏免疫球蛋白、补体受体和Fc受体，同时存在绵羊红细胞受体和T细胞抗原，据此将其归类为胸腺来源。采用定量显微光度免疫放射自显影技术，在细胞水平上测定表面抗原密度，结果如下：（1）与正常T淋巴细胞相比，白血病细胞上T抗原位点的密度较低。（2）白血病淋巴细胞的T抗原密度在细胞间变化较小，在直方图中形成一个均匀的峰值。（3）在残留的T抗原阴性细胞上显示出正常B淋巴细胞的Ig密度。通过直接免疫荧光和过氧化物酶标记抗体的电子显微镜检查对结果进行了定性确认。此外，采用定量微量补体结合试验检测表面抗原，结果显示抗T细胞抗体与补体的结合减少，白血病淋巴细胞上无抗球蛋白结合。由于在白血病T淋巴细胞中未发现T抗原浓度正常的淋巴细胞，因此推测缺乏正常T细胞。关于B细胞型和T细胞型慢性淋巴细胞白血病细胞表面标志物减少及分布改变的研究结果，作为涉及其克隆起源的进一步论据进行了讨论。
