从噬菌体展示文库中分离棘阿米巴特异性抗体。
Isolation of Acanthamoeba-specific antibodies from a bacteriophage display library.
作者信息
Khan N A, Greenman J, Topping K P, Hough V C, Temple G S, Paget T A
机构信息
Department of Biological Science, The University of Hull, Hull, United Kingdom HU6 7RX.
出版信息
J Clin Microbiol. 2000 Jun;38(6):2374-7. doi: 10.1128/JCM.38.6.2374-2377.2000.
Abstract
Acanthamoeba causes opportunistic eye infections in humans, which can lead to severe keratitis and may ultimately result in blindness. Current methods for identifying this organism rely on culture and microscopy. In this paper, we describe the isolation of antibody fragments that can be used for the unequivocal identification of Acanthamoeba. A bacteriophage antibody display library was used to isolate antibody fragments that bind specifically to Acanthamoeba. Individual clones were studied by enzyme-linked immunosorbent assay, flow cytometry, and immunofluorescence. Four antibody clones that specifically bind to Acanthamoeba spp. were identified.
摘要
棘阿米巴可引起人类机会性眼部感染，导致严重角膜炎，最终可能导致失明。目前鉴定这种微生物的方法依赖于培养和显微镜检查。在本文中，我们描述了可用于明确鉴定棘阿米巴的抗体片段的分离。使用噬菌体抗体展示文库分离与棘阿米巴特异性结合的抗体片段。通过酶联免疫吸附测定、流式细胞术和免疫荧光对单个克隆进行研究。鉴定出四个与棘阿米巴属特异性结合的抗体克隆。
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