悬钩子属植物细胞悬浮培养物中苯亚甲基丙酮和树莓酮的产生与诱导

Production and elicitation of benzalacetone and the raspberry ketone in cell suspension cultures of Rubus idaeus.

作者信息

Pedapudi S, Chin C K, Pedersen H

机构信息

Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, Piscataway New Jersey 08854-8058, USA.

出版信息

Biotechnol Prog. 2000 May-Jun;16(3):346-9. doi: 10.1021/bp000033i.

DOI:10.1021/bp000033i

PMID:10835233

Abstract

Production levels of p-coumaric acid (p-CA), p-hydroxyphenylbut-3-ene-2-one (benzalacetone), and p-hydroxyphenyl-2-butanone (raspberry ketone) were measured in raspberry cell suspension cultures to investigate metabolite dynamics in a short (two-step) pathway. Intracellular concentrations of benzalacetone and the raspberry ketone fluctuated during the time course of a normal batch culture cycle but showed higher levels during periods of rapid growth. Cells elicited with the signal coupler methyl jasmonate yielded a 2- to 3-fold increase in metabolite concentrations after 24 h. The results suggest that raspberry ketone production is rapidly inducible during periods of high carbohydrate utilization. It is not an end product, however, and undergoes conversion to subsequent metabolites.

摘要

在树莓细胞悬浮培养物中测定了对香豆酸（p-CA）、对羟基苯基丁-3-烯-2-酮（苯亚甲基丙酮）和对羟基苯基-2-丁酮（树莓酮）的产量，以研究短（两步）途径中的代谢物动态。在正常分批培养周期的时间进程中，苯亚甲基丙酮和树莓酮的细胞内浓度有所波动，但在快速生长阶段显示出较高水平。用信号偶联剂茉莉酸甲酯诱导的细胞在24小时后代谢物浓度增加了2至3倍。结果表明，在高碳水化合物利用期间，树莓酮的产量可迅速诱导。然而，它不是终产物，会转化为后续代谢物。