Medeiros R G, Soffner M L, Thomé J A, Cacais A O, Estelles R S, Salles B C, Ferreira H M, Lucena Neto S A, Silva F G, Filho E X
Laboratório de Enzimologia, Departamento de Biologia Celular, Universidade de Brasília, CEP 70910-900, Brasil.
Biotechnol Prog. 2000 May-Jun;16(3):522-4. doi: 10.1021/bp0000398.
Trichoderma harzianum strains T4 and T6, Acrophialophora nainiana, and Humicola grisea var. thermoidea were screened for their ability to produce carbohydrate-degrading enzyme activities in a medium containing banana plant residue as the carbon source. The best balance of enzyme activities was obtained from cultures of H. grisea var. thermoidea. Xylanase activity from crude extract of A. nainiana had a maximum activity at pH 5.5-7.0 and a temperature range of 50-55 degrees C. It was stable up to 55 degrees C at pH 7.0 for at least 2 h. The fungi were also able to produce xylanase and pectinase activities when grown on extractives as substrate.
对哈茨木霉T4和T6菌株、矮小顶孢霉以及嗜热灰腐质霉进行了筛选,以检测它们在以香蕉植物残渣作为碳源的培养基中产生碳水化合物降解酶活性的能力。从嗜热灰腐质霉培养物中获得了最佳的酶活性平衡。矮小顶孢霉粗提物中的木聚糖酶活性在pH 5.5 - 7.0以及温度范围为50 - 55摄氏度时达到最大值。在pH 7.0时,该酶在高达55摄氏度的温度下至少2小时内保持稳定。这些真菌在以提取物为底物生长时也能够产生木聚糖酶和果胶酶活性。