Teissié J, Eynard N, Gabriel B, Rols MP
IPBS UPR 9062 CNRS, 118 Route de Narbonne, 31062 Toulouse, France
Adv Drug Deliv Rev. 1999 Jan 4;35(1):3-19. doi: 10.1016/s0169-409x(98)00060-x.
A position dependent modulation of the membrane potential difference is induced when an electric field is applied to a cell. When cells are submitted to short lived electric field pulses with an overcritical intensity, a local membrane alteration is induced, which may reseal. Its molecular definition remains unknown. A free exchange of hydrophilic molecules takes place across the membrane. A leakage of cytosolic metabolites is present. However, a loading of polar drugs into the cytoplasm is obtained. A short description of the processes affecting the cell membrane organization is given. Lipids appear as the primary target of the field effect as in the case of liposomes. Nevertheless membrane proteins appear to be affected by a direct or by a back effect. The permeabilized state is long lived. The cell metabolism plays indeed a critical role in the recovery. The cell viability can be nevertheless preserved.
当向细胞施加电场时，会诱导膜电位差的位置依赖性调制。当细胞受到强度超过临界值的短暂电场脉冲作用时，会诱导局部膜改变，这种改变可能会重新封闭。其分子定义仍然未知。亲水分子可在膜上自由交换。存在细胞溶质代谢物的泄漏。然而，极性药物可被载入细胞质。文中给出了影响细胞膜组织过程的简要描述。与脂质体的情况一样，脂质似乎是场效应的主要靶点。然而，膜蛋白似乎会受到直接或反向效应的影响。通透状态持续时间长。细胞代谢在恢复过程中确实起着关键作用。然而，细胞活力仍可得以保留。
