左旋咪唑对小鼠淋巴细胞培养物的体外刺激作用。
In vitro stimulation of murine lymphoid cell cultures by levamisole.
作者信息
Merluzzi V J, Badger A M, Kaiser C W, Cooperband S R
出版信息
Clin Exp Immunol. 1975 Dec;22(3):486-92.
Levamisole has been reported to act as an immunological adjuvant. Experiments reported here on the effect of this agent on a variety of murine lymphoid culture systems were designed to gain an insight into its mechanism of action. We have found levamisole to be a weak mitogen for mouse spleen cells producing a dose related response which peaks at 48 hr in culture. The drug acted to augment the response of spleen cells to sub-optimal concentrations of concanavalin A, but had no unusual effect on the lipopolysaccharide stimulation of B-cell DNA synthesis in vitro. Levamisole was directly stimulatory on enriched T-cell populations and was found to have two actions: (1) to stimulate a subpopulation of T cells and (2) to augment the response of suboptimal mitogen concentrations of concanavalin A. In addition, we have found that murine thymocytes stimulated by concanavalin A were greatly potentiated in the presence of levamisole, but this population of cells could not be stimulated directly by the drug.
据报道，左旋咪唑可作为一种免疫佐剂。本文所报道的关于该药物对多种小鼠淋巴细胞培养系统作用的实验，旨在深入了解其作用机制。我们发现左旋咪唑是一种对小鼠脾细胞作用较弱的促有丝分裂原，可产生剂量相关反应，在培养48小时时达到峰值。该药物可增强脾细胞对次优浓度刀豆蛋白A的反应，但对体外脂多糖刺激B细胞DNA合成没有异常影响。左旋咪唑对富集的T细胞群体有直接刺激作用，且发现有两种作用：（1）刺激T细胞亚群；（2）增强次优浓度刀豆蛋白A促有丝分裂原的反应。此外，我们还发现，在左旋咪唑存在的情况下，刀豆蛋白A刺激的小鼠胸腺细胞的反应大大增强，但该细胞群体不能被该药物直接刺激。
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