Hersey P, Ho K, Werkmeister J, Abele U
Clin Exp Immunol. 1981 Nov;46(2):340-9.
The action of levamisole on immunoglobulin production was investigated in pokeweed mitogen-stimulated cultures of B and T cells. Immunoglobulin production was measured by nephelometric methods using specific antisera to IgA, IgG and IgM. Levamisole was shown to have no effect on B lymphocytes but pretreatment of T lymphocytes with levamisole resulted in an increase in immunoglobulin production which was maximal at 10(-6) M. The latter increase appeared to be due to an effect on suppressor T cells in that pretreatment of T cells depleted of suppressor cells (by irradiation or removal of T cells with receptors with IgG) did not result in an increase in immunoglobulin production. Similar effects of levamisole were demonstrated against suppressor cells induced by concanavalin A in vitro and against spontaneous suppressor cell activity in vivo. The effect of levamisole and irradiation on suppressor cell activity against particular classes of immunoglobulin varied between individuals. Suppressor cell activity against IgA production alone was demonstrated in six of 17 subjects whereas in the remainder, immunoglobulin classes. The implications of this action of levamisole for its effects in vivo are unknown, but the results appear consistent with many of the observed effects of the drug. Selection of patients with diseases associated with high suppressor cell activity may lead to more effective use of the drug.
在美洲商陆丝裂原刺激的B细胞和T细胞培养物中研究了左旋咪唑对免疫球蛋白产生的作用。使用针对IgA、IgG和IgM的特异性抗血清，通过比浊法测量免疫球蛋白的产生。结果显示左旋咪唑对B淋巴细胞无作用，但用左旋咪唑预处理T淋巴细胞会导致免疫球蛋白产生增加，在10（-6）M时达到最大值。后一种增加似乎是由于对抑制性T细胞的作用，因为对去除了抑制性细胞（通过照射或用带有IgG受体的T细胞去除）的T细胞进行预处理不会导致免疫球蛋白产生增加。在体外，左旋咪唑对刀豆蛋白A诱导的抑制性细胞以及在体内对自发抑制性细胞活性都表现出类似的作用。左旋咪唑和照射对针对特定免疫球蛋白类别的抑制性细胞活性的影响在个体之间有所不同。在17名受试者中的6名中仅显示出对IgA产生的抑制性细胞活性，而在其余受试者中则针对其他免疫球蛋白类别。左旋咪唑的这种作用对其体内效应的影响尚不清楚，但结果似乎与该药物许多观察到的效应一致。选择患有与高抑制性细胞活性相关疾病的患者可能会导致更有效地使用该药物。
