Carsten A L, Bond V P, Thompson K
Int J Radiat Biol Relat Stud Phys Chem Med. 1976 Jan;29(1):65-70. doi: 10.1080/09553007614551561.
The spleen-colony technique has been used for determining the relative biological effectiveness (r.b.e.) for several energies of neutron radiation. Donor mice were exposed for fission and accelerator-generated neutrons at a variety of doses and energies. Immediately after exposure, donor bone-marrow was removed from the hind legs, and standard amounts were injected intravenously into lethally X-irradiated recipients. After 7 days the recipients spleens were evaluated for surface colonies. Dose-response curves were obtained for each type of radiation and the Do was determined. The neutron r.b.e. values from the Do compared with 250kVp X-rays were: reactor 1.58, 252Cf 1:59, and accelerator varied from 2.85 at 1.0 Mev to 0.85 at 13.4 MeV.
脾集落技术已被用于测定几种能量的中子辐射的相对生物效应（r.b.e.）。供体小鼠接受了不同剂量和能量的裂变中子和加速器产生的中子照射。照射后立即从供体小鼠的后腿取出骨髓，并将标准量静脉注射到经致死性X射线照射的受体小鼠体内。7天后，对受体小鼠的脾脏表面集落进行评估。获得了每种辐射类型的剂量-反应曲线，并确定了Do值。与250kVp X射线相比，通过Do得出的中子r.b.e.值为：反应堆中子1.58，252Cf中子1.59，加速器产生的中子在1.0兆电子伏时为2.85，在13.4兆电子伏时为0.85。
