Etlinger H M, Hodgins H O, Chiller J M
J Immunol. 1976 Jun;116(6):1547-53.
Leukocytes from the various lymphoid tissues of rainbow trout (RBT) were tested for their capacity to respond to the lymphocyte mitogens concanavalin A (Con A), lipopolysaccharide (LPS), and purified protein derivative of tuberculin (PPD). Thymocytes responded to Con A but not to LPS or PPD. In contrast, leukocytes from anterior kidney were stimulated with LPS but not with Con A or PPD. Cells from spleen and peripheral blood were stimulated by each mitogen. However, the degree of stimulation at optimally stimulatory concentrations of each mitogen was distinctive. The finding that the patterns of mitogenic responses of cells from each tissue were significantly different suggested that there is lymphoid heterogeneity in the RBT with a unique tissue distribution. The species source of serum utilized as a medium supplement appeared to be capable of markedly affecting mitogenesis. Thus, LPS and PPD stimulation occurred in medium supplemented with rainbow trout serum (RBTS). On the other hand, LPS and PPD stimulation was not observed in medium supplemented with fetal bovine serum (FBS), with the exception of peripheral blood leukocytes which were stimulated by LPS in culture medium supplemented with FBS. Con A stimulated leukocytes from each lymphoid tissue in medium supplemented with RBTS and, with the exception of cells from anterior kidney, also stimulated cells from each tissue in medium supplemented with FBS. The kinetic profiles of the responses of peripheral blood leukocytes to Con A, LPS, and PPD suggested that the extent as well as the time required for maximal stimulation was dependent on the dose of mitogen.
对虹鳟（RBT）各种淋巴组织中的白细胞进行检测，以评估它们对淋巴细胞有丝分裂原刀豆蛋白A（Con A）、脂多糖（LPS）和结核菌素纯蛋白衍生物（PPD）的反应能力。胸腺细胞对Con A有反应，但对LPS或PPD无反应。相比之下，前肾白细胞受到LPS刺激，但不受Con A或PPD刺激。脾脏和外周血中的细胞受到每种有丝分裂原的刺激。然而，在每种有丝分裂原的最佳刺激浓度下，刺激程度各不相同。每个组织的细胞有丝分裂反应模式存在显著差异，这一发现表明虹鳟存在淋巴异质性，且具有独特的组织分布。用作培养基补充剂的血清的物种来源似乎能够显著影响有丝分裂。因此，在补充虹鳟血清（RBTS）的培养基中发生LPS和PPD刺激。另一方面，在补充胎牛血清（FBS）的培养基中未观察到LPS和PPD刺激，但在补充FBS的培养基中，外周血白细胞受到LPS刺激的情况除外。Con A在补充RBTS的培养基中刺激每个淋巴组织的白细胞，除前肾细胞外，在补充FBS的培养基中也刺激每个组织的细胞。外周血白细胞对Con A、LPS和PPD反应的动力学曲线表明，最大刺激所需的程度和时间取决于有丝分裂原的剂量。
