Banaszewski J, Szmeja Z, Szyfter W, Szyfter K, Baranczewski P, Möller L
Department of Otolaryngology, University School of Medicine, Poznań, Poland.
Eur Arch Otorhinolaryngol. 2000;257(3):149-53. doi: 10.1007/s004050050212.
Epidemiological studies have confirmed the correlation between tobacco smoking, environmental pollution and the incidence of cancers of the respiratory tract. The occurrence of laryngeal cancer in Poland is relatively high compared to other European countries. Since 1969 the mortality related to larynx cancer appears to be increasing. Tobacco smoke contains an abundance of such carcinogenic compounds as polycyclic aromatic hydrocarbons (PAH), aromatic amines and N-nitrosoamines, which can react with DNA and form adducts. We analyzed aromatic DNA adducts in laryngeal tissues from patients with primary laryngeal, which was confirmed histopathologically to be squamous cell carcinoma. The group consisted of 33 patients (5 women and 28 men). Total laryngectomy was performed in patients. A detergentphenol method was used for DNA isolation. Aromatic DNA adducts were analyzed by a 32P-postlabelling technique with butanol extraction and high performance liquid chromatography. The presence of aromatic DNA adducts was demonstrated in all tissues. Large interindividual differences of DNA adduct levels were seen in each tissue studied. There was a higher mean level of DNA adducts in interarytenoid area non-tumors (51.96/10(8) +/- 91.71 NN) than in non-tumor tissue elsewhere (46.91/10(8) +/- 46.36 NN) and tumor tissue (43.52/10(8) +/- 45.88 NN). Adduct levels were correlated with age, sex, cigarette smoking and TNM stage.
流行病学研究已证实吸烟、环境污染与呼吸道癌症发病率之间的关联。与其他欧洲国家相比，波兰喉癌的发病率相对较高。自1969年以来，与喉癌相关的死亡率似乎一直在上升。烟草烟雾中含有大量致癌化合物，如多环芳烃（PAH）、芳香胺和N-亚硝胺，它们可与DNA反应并形成加合物。我们分析了原发性喉癌患者喉组织中的芳香族DNA加合物，这些患者经组织病理学确诊为鳞状细胞癌。该组由33名患者组成（5名女性和28名男性）。患者均接受了全喉切除术。采用去污剂-酚法分离DNA。通过丁醇萃取和高效液相色谱的32P后标记技术分析芳香族DNA加合物。在所有组织中均证实存在芳香族DNA加合物。在所研究的每个组织中，DNA加合物水平存在较大的个体差异。杓间区非肿瘤组织中的DNA加合物平均水平（51.96/10(8) +/- 91.71 NN）高于其他部位的非肿瘤组织（46.91/10(8) +/- 46.36 NN）和肿瘤组织（43.52/10(8) +/- 45.88 NN）。加合物水平与年龄、性别、吸烟和TNM分期相关。
