Pabiszczak M, Banaszewski J, Szmeja Z, Szyfter K, Szyfter W
Klinika Otolaryngologii Katedry Chorób Ucha, Nosa, Gardła i Krtani AM im. K. Marcinkowskiego w Poznaniu.
Otolaryngol Pol. 2001;55(5):551-4.
The results concerning examination of DNA adducts in oral (23 patients), pharyngeal (23 patients) and larynx cancer (10 patients) subjects are presented. DNA adduct levels were compared in respect to anatomical structure (primary tumour location), number of cigarettes smoked, TNM stage, and age of patients. DNA was isolated from removed tissue (tumour and non-tumour surrounding tissue) using detergent/phenol extraction. 32P-postlabelling assay including nuclease P1-enhancement modification was applied. Aromatic DNA adducts were found in all studied tissues. Total DNA adduct levels (tumour and non-tumour tissues) was lowest in larynx cancer, higher in oral cancer and highest in pharyngeal cancer. There were no influence of age into formation of DNA adducts. The higher level of DNA adducts was found in tumour tissue of oral cancer in the group of smokers with metastasis into lymph nodes.
本文展示了对口腔癌（23例患者）、咽癌（23例患者）和喉癌（10例患者）受试者DNA加合物检测的结果。比较了DNA加合物水平与解剖结构（原发肿瘤位置）、吸烟数量、TNM分期及患者年龄之间的关系。使用去污剂/苯酚提取法从切除组织（肿瘤组织及肿瘤周围的非肿瘤组织）中分离DNA。应用了包括核酸酶P1增强修饰的32P后标记测定法。在所有研究组织中均发现了芳香族DNA加合物。总DNA加合物水平（肿瘤组织和非肿瘤组织）在喉癌中最低，在口腔癌中较高，在咽癌中最高。年龄对DNA加合物的形成没有影响。在有淋巴结转移的吸烟组口腔癌肿瘤组织中发现了较高水平的DNA加合物。
