Szyfter K, Hemminki K, Szyfter W, Szmeja Z, Banaszewski J, Yang K
Center for Nutrition and Toxicology, Karolinska Institute, Huddinge, Sweden.
Carcinogenesis. 1994 Oct;15(10):2195-9. doi: 10.1093/carcin/15.10.2195.
Larynx cancer is strongly associated with tobacco smoking. The objective of this work was an analysis of aromatic DNA adducts in tumour and non-tumour larynx cells by means of the 32P-postlabelling method. Peripheral blood leukocytes were used as a reference tissue. The presence of aromatic DNA adducts was demonstrated in all the studied tissues obtained after surgery of larynx tumours. The highest level of DNA adducts was found in larynx tumour cells, followed by non-tumour larynx cells, which exceeded that found in leukocytes almost 2.5 times. Large interindividual differences were detected between subjects. The adduct level in tumour/non-tumour correlated only moderately. However a high correlation was found between the level of DNA adducts in larynx (tumour and non-tumour) cells and that in leukocytes.
喉癌与吸烟密切相关。本研究的目的是通过³²P后标记法分析肿瘤性和非肿瘤性喉细胞中的芳香族DNA加合物。外周血白细胞用作参考组织。在喉肿瘤手术后获得的所有研究组织中均证实存在芳香族DNA加合物。喉肿瘤细胞中发现的DNA加合物水平最高，其次是非肿瘤性喉细胞，其比白细胞中的水平高出近2.5倍。受试者之间存在较大的个体差异。肿瘤/非肿瘤中的加合物水平仅呈中度相关。然而，在喉（肿瘤性和非肿瘤性）细胞与白细胞中的DNA加合物水平之间发现高度相关。
