Tresoldi A T, Padoveze M C, Trabasso P, Veiga J F, Marba S T, von Nowakonski A, Branchini M L
Nosocomial Infection Control Committee, Faculty of Medical Sciences, State University of Campinas, Brazil.
Am J Infect Control. 2000 Jun;28(3):258-61. doi: 10.1067/mic.2000.105286.
The study aimed to investigate an outbreak caused by Enterobacter cloacae in a neonate intensive care unit.
A descriptive study of an outbreak of sepsis in high-risk neonates was used.
The study was set in a tertiary care university teaching hospital.
The patients were 11 neonates infected with Enterobacter cloacae whose symptoms and signs of sepsis developed during a 16-hour period. All but one neonate received parenteral nutrition. Isolates from blood cultures, in-use parenteral nutrition solutions, and control aliquots of parenteral nutrition solution were typed by pulsed-field gel electrophoresis.
Enterobacter cloacae was found in the refrigerated aliquots of parenteral nutrition solution, in blood cultures from infected newborns, and from in-use parenteral nutrition solutions. All these strains of Enterobacter cloacae had the same antibiotic susceptibility pattern and the same genomic DNA profile. The strain isolated from the one patient who did not receive parenteral nutrition presented a different susceptibility profile and genotype.
The source of the nosocomial sepsis was the parenteral nutrition solution in 10 neonates. This contamination apparently occurred during preparation of the parenteral solution.
本研究旨在调查新生儿重症监护病房由阴沟肠杆菌引起的一次暴发。
采用对高危新生儿败血症暴发进行描述性研究。
研究在一家三级护理大学教学医院开展。
11名感染阴沟肠杆菌的新生儿，其败血症症状和体征在16小时内出现。除1名新生儿外，其余均接受肠外营养。对血培养物、在用肠外营养溶液以及肠外营养溶液对照等分试样中的分离株进行脉冲场凝胶电泳分型。
在肠外营养溶液的冷藏等分试样、感染新生儿的血培养物以及在用肠外营养溶液中均发现阴沟肠杆菌。所有这些阴沟肠杆菌菌株具有相同的抗生素敏感性模式和相同的基因组DNA图谱。从1名未接受肠外营养的患者分离出的菌株呈现不同的敏感性谱和基因型。
10名新生儿医院内败血症的来源是肠外营养溶液。这种污染显然发生在肠外溶液的制备过程中。
