Johnsson C, Hällgren R, Tufveson G
Departments of Transplantation Surgery and Internal Medicine, University Hospital, Uppsala, Sweden.
Surgery. 2000 Jun;127(6):650-8. doi: 10.1067/msy.2000.106587.
The connective tissue component hyaluronan is accumulated locally in the damaged tissue during various inflammatory conditions. Owing to the strong water-binding capacity of this glycosaminoglycan, increased tissue content of hyaluronan is paralleled by the development of interstitial edema. The aim with the current experiment was to investigate whether hyaluronan is accumulated in acute pancreatitis and if increased levels of hyaluronan can be correlated to the inflammation of the pancreatic tissue.
Acute pancreatitis was induced in Sprague-Dawley rats by the administration of supramaximal doses of the cholecystokinin analogue caerulein. The animals were followed for 5 hours (n = 4), 24 hours (n = 6), or 48 hours (n = 5), and the pancreata were then investigated for hyaluronan and water content, hyaluronan distribution, general morphology and the presence of CD44-positive cells, macrophages, and T lymphocytes.
Hyaluronan accumulated in the edematous interstitium during acute pancreatitis. Twenty-four hours after the induction of pancreatitis, the hyaluronan content of the pancreata had increased by more than 100%. Simultaneously, CD44-positive cells infiltrated the tissue. However, no correlation between hyaluronan and water was seen at any time point.
This study shows that acute pancreatitis is associated with a strong but transient increase in interstitial hyaluronan and an infiltration of CD44-positive cells located mainly in the same region as the accumulated hyaluronan.
在各种炎症状态下，结缔组织成分透明质酸会在受损组织中局部蓄积。由于这种糖胺聚糖具有强大的水结合能力，透明质酸组织含量增加的同时会伴有间质水肿的发展。本实验的目的是研究透明质酸是否会在急性胰腺炎中蓄积，以及透明质酸水平升高是否与胰腺组织炎症相关。
通过给予超最大剂量的胆囊收缩素类似物蛙皮素，在Sprague-Dawley大鼠中诱导急性胰腺炎。对动物进行5小时（n = 4）、24小时（n = 6）或48小时（n = 5）的观察，然后对胰腺进行透明质酸和水分含量、透明质酸分布、大体形态以及CD44阳性细胞、巨噬细胞和T淋巴细胞存在情况的研究。
在急性胰腺炎期间，透明质酸在水肿的间质中蓄积。胰腺炎诱导后24小时，胰腺的透明质酸含量增加了100%以上。同时，CD44阳性细胞浸润组织。然而，在任何时间点均未观察到透明质酸与水分之间的相关性。
本研究表明，急性胰腺炎与间质透明质酸的强烈但短暂增加以及主要位于与蓄积透明质酸相同区域的CD44阳性细胞浸润有关。
