Department of Medical Cell Biology, Biomedical Centre, Uppsala University, Uppsala, Sweden.
Scand J Immunol. 2010 Nov;72(5):416-24. doi: 10.1111/j.1365-3083.2010.02455.x.
Hyaluronan is known to accumulate in tissues during inflammatory diseases associated with graft implantation and rejection of organ allografts. The aim was to evaluate whether hyaluronidase treatment affected hyaluronan content and blood perfusion in graft pancreatitis. Syngeneic rat pancreatic-duodenal transplantations were performed. Two days later blood flow measurements were made with a microsphere technique in both grafted and endogenous pancreas in animals treated with daily injections of vehicle or hyaluronidase (20.000 U/kg). Non-transplanted rats served as controls. Also, samples for analysis of hyaluronan and water content were taken. The hyaluronan content of the pancreatic graft was increased after transplantation. Hyaluronidase treatment markedly reduced total pancreatic and islet blood flow in both grafted and endogenous pancreas, whereas duodenum blood flow was unaffected. No blood flow effects were seen in non-transplanted control rats. Hyaluronan content was increased in the grafted pancreas, but hyaluronidase treatment decreased it to levels comparable to those of the endogenous gland. There were no differences in hyaluronan content in the endogenous pancreases of transplanted and non-transplanted rats. Graft pancreatitis after rat pancreas transplantation is associated with an increased hyaluronan content, which can be reduced by treatment with hyaluronidase. Hyaluronidase treatment of the graft recipients effected a 50% reduction in total pancreatic and islet blood flow in the graft, as well as in the endogenous pancreas. The functional importance of this is at present unknown.
透明质酸在与移植物植入和同种异体器官移植物排斥相关的炎症性疾病中已知会在组织中积累。目的是评估透明质酸酶治疗是否会影响移植物胰腺炎中的透明质酸含量和血液灌注。进行了同基因大鼠胰十二指肠移植。两天后，用微球技术在接受每日注射载体或透明质酸酶（20,000 U/kg）的移植和内源性胰腺中测量血流。未移植的大鼠作为对照。还采集了用于分析透明质酸和水含量的样本。移植后胰腺移植物的透明质酸含量增加。透明质酸酶治疗明显降低了移植和内源性胰腺的总胰腺和胰岛血流，而十二指肠血流不受影响。未移植的对照大鼠未观察到血流效应。移植胰腺中的透明质酸含量增加，但透明质酸酶治疗将其降低至与内源性腺体相当的水平。移植和未移植大鼠的内源性胰腺中的透明质酸含量没有差异。大鼠胰腺移植后的移植胰腺炎与透明质酸含量增加有关，透明质酸酶治疗可降低其含量。移植物受者的透明质酸酶治疗使移植和内源性胰腺中的总胰腺和胰岛血流减少了 50%。目前尚不清楚这种功能的重要性。
