流感嗜血杆菌三种Rec突变体的特性相似性及图谱分析
Similarity in properties and mapping of three Rec mutants of Haemophilus influenzae.
作者信息
Kooistra J, Setlow J K
出版信息
J Bacteriol. 1976 Jul;127(1):327-33. doi: 10.1128/jb.127.1.327-333.1976.
Abstract
Three Rec- mutants of Haemophilus influenzae have been studied with respect to their transformability, ultraviolet and mitomycin C sensitivities, spontaneous and ultraviolet-induced deoxyribonucleic acid breakdown, inducibility of lysogens, and the linkage of the three mutations to a streptomycin resistance marker. The data indicate that the three mutations cause the same phenotypic changes, and that they are all on the same gene. Transformability of the mutants is different when two different media are used for competence development, although transformability with the two competence methods is not different in a Rec- strain that is mutant at another gene.
摘要
针对流感嗜血杆菌的三个Rec-突变体，研究了它们的转化能力、对紫外线和丝裂霉素C的敏感性、自发及紫外线诱导的脱氧核糖核酸降解、溶原菌的诱导性，以及这三个突变与链霉素抗性标记的连锁关系。数据表明，这三个突变导致相同的表型变化，且它们都位于同一个基因上。当使用两种不同培养基进行感受态发育时，突变体的转化能力有所不同，尽管在另一个基因发生突变的Rec-菌株中，两种感受态方法的转化能力并无差异。
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