Stuy J H
J Bacteriol. 1980 Jun;142(3):925-30. doi: 10.1128/jb.142.3.925-930.1980.
Twenty-three highly antibiotic-resistant strains of Haemophilus influenzae and two of Haemophilus parainfluenzae without detectable large plasmids were examined for conjugative transfer of their resistance to H. influenzae strain Rd or to other strains. Very inefficient transfer was observed for 18 H. influenzae strains and 1 H. parainfluenzae strain. All H. influenzae transcipients carried a large plasmid, and they were in turn efficient donors of their resistances in standard conjugation crosses with isogenic recipients. This was not seen for the H. parainfluenzae transcipients. It is concluded that most of the original antibiotic-resistant cultures carried an integrated conjugative R plasmid which had been excised in a few cells in each population. It was these cells which transferred resistance in the primary crosses.
对23株高度耐抗生素的流感嗜血杆菌和2株未检测到大型质粒的副流感嗜血杆菌进行检测，以观察它们的耐药性向流感嗜血杆菌Rd菌株或其他菌株的接合转移情况。在18株流感嗜血杆菌和1株副流感嗜血杆菌中观察到非常低效的转移。所有流感嗜血杆菌转导子都携带一个大型质粒，并且在与同基因受体的标准接合杂交中，它们反过来又是其耐药性的高效供体。副流感嗜血杆菌转导子则未出现这种情况。得出的结论是，大多数原始耐抗生素培养物携带一个整合的接合性R质粒，该质粒在每个群体的少数细胞中已被切除。正是这些细胞在初次杂交中转移了耐药性。
