流感嗜血杆菌中累加基因转化的机制。
Mechanism of additive genetic transformation in Haemophilus influenzae.
作者信息
Stuy J H
出版信息
J Bacteriol. 1980 Dec;144(3):999-1002. doi: 10.1128/jb.144.3.999-1002.1980.
Transforming deoxyribonucleic acid (DNA) preparations from Haemophilus influenzae Rd strains carrying a chromosomally integrated, conjugative, antibiotic resistance transfer (R) plasmid were exposed to ultraviolet radiation and then assayed for antibiotic resistance transfer on sensitive wild-type Rd competent suspensions and on similar suspensions of a uvr-1 mutant unable to excise pyrimidine dimers. No host cell reactivation of resistance transfer (DNA repair) was observed. Parallel experiments with ethanol-precipitated, heated, free R plasmid DNA preparations gave much higher survival when assayed on the wild-type strain compared to the survival on the uvr-1 strain. These observations indicate that additive genetic transformation (in this case, the addition of the integrated R plasmid to the recipient genome) involves single-strand insertion.
将携带染色体整合的、可接合的抗生素抗性转移（R）质粒的流感嗜血杆菌Rd菌株的脱氧核糖核酸（DNA）制剂暴露于紫外线辐射，然后在敏感的野生型Rd感受态悬浮液和不能切除嘧啶二聚体的uvr - 1突变体的类似悬浮液上检测抗生素抗性转移。未观察到抗性转移的宿主细胞再活化（DNA修复）。与在uvr - 1菌株上的存活率相比，用乙醇沉淀、加热的游离R质粒DNA制剂进行的平行实验在野生型菌株上检测时存活率要高得多。这些观察结果表明，附加遗传转化（在这种情况下，将整合的R质粒添加到受体基因组中）涉及单链插入。
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