Sasaki A, Nakazato Y, Ogawa A, Sugihara S
Department of Pathology, Gunma University School of Medicine, Maebashi, Japan.
Pathol Int. 1996 Jan;46(1):15-23. doi: 10.1111/j.1440-1827.1996.tb03528.x.
The immunophenotype of perivascular cells (PC) in temporal lobe tissues obtained at autopsy in 48 patients (aged 41-88 years) was characterized using light and electron microscopic immunocytochemistry with a variety of antibodies. In all cases studied, PC bearing CD11c (Ki-M1P) and CD68 (KP1) were distributed throughout the temporal cortex. In addition to Ki-M1P and KP1, the monoclonal antibodies against major histocompatibility complex (MHC) class II antigen (Ag) (LN-3, CR3.43), anti-leucocyte common antigen (LCA), LN-5, Mac 387 were all found in PC with variable immunoreactivity. In contrast, LN-1 and OPD4 were not found in PC, although the former showed nearly constant staining of resting microglia. Semiquantitative analysis disclosed differences in the numbers of cells labeled with the markers in the 21 normal brains (Ki-M1P > KP1 >> LCA, LN-3, LN-5 >> Mac 387). Ultrastructurally, immunoreactivity for Ki-M1P, KP1, and LN-3 was observed in PC with cytoplasm containing dense lysosomal bodies. In brains from patients with Alzheimer's type dementia, PC were seen in the wall of beta-amyloid protein-positive small vessels. However, there was no definite alteration of antigenicity in PC from AD brains compared with those from normal brains. The immunophenotype of PC was similar to that of macrophages, which were observed in the perivascular spaces and the leptomeninges in some normal and diseased brains. In contrast with PC, however, macrophages showed high incidence of labeling for some macrophage markers LN-5 and Mac 387. These findings demonstrate that PC may be a normal constituent of the adult human brain with a variable expression of monocyte/macrophages markers and MHC class II Ag and that PC could be distinguished from resting microglia by their morphology and by their immunophenotype.
对48例尸检获得的颞叶组织（年龄41 - 88岁）中的血管周围细胞（PC）进行免疫表型分析，采用光镜和电镜免疫细胞化学技术，使用多种抗体。在所有研究病例中，表达CD11c（Ki - M1P）和CD68（KP1）的PC分布于整个颞叶皮质。除了Ki - M1P和KP1外，抗主要组织相容性复合体（MHC）II类抗原（Ag）（LN - 3、CR3.43）、抗白细胞共同抗原（LCA）、LN - 5、Mac 387等单克隆抗体在PC中均有不同程度的免疫反应性。相反，尽管LN - 1对静息小胶质细胞显示出几乎恒定的染色，但在PC中未发现LN - 1和OPD4。半定量分析显示，在21例正常脑标本中，标记细胞数量存在差异（Ki - M1P > KP1 >> LCA、LN - 3、LN - 5 >> Mac 387）。超微结构上，在细胞质中含有致密溶酶体的PC中观察到Ki - M1P、KP1和LN - 3的免疫反应性。在阿尔茨海默病型痴呆患者的脑中，在β - 淀粉样蛋白阳性小血管壁中可见PC。然而，与正常脑相比，AD脑中PC的抗原性没有明显改变。PC的免疫表型与巨噬细胞相似，在一些正常和患病脑中，巨噬细胞可见于血管周围间隙和软脑膜。然而，与PC不同的是，巨噬细胞对某些巨噬细胞标记物LN - 5和Mac 387的标记发生率较高。这些发现表明，PC可能是成人大脑的正常组成部分，具有单核细胞/巨噬细胞标记物和MHC II类Ag的可变表达，并且PC可以通过其形态和免疫表型与静息小胶质细胞区分开来。
