Graeber M B, Streit W J, Büringer D, Sparks D L, Kreutzberg G W
Center for Neurologic Diseases, Brigham and Women's Hospital, Boston, Massachusetts.
J Neuropathol Exp Neurol. 1992 May;51(3):303-11. doi: 10.1097/00005072-199205000-00009.
The expression of major histocompatibility complex (MHC) class I and II antigens was studied in surgical and postmortem brain biopsy tissue using light and electron microscopic immunocytochemistry. In addition, monoclonal antibodies directed against human macrophages (EBM11) and alpha-smooth muscle actin were applied. It is shown that blood vessel-associated MHC class II immunoreactivity in histologically normal human brain can be localized to a distinct class of cells, termed perivascular cells, which share macrophage but not smooth muscle cell antigen. This immunophenotype, the location in the perivascular space as well as the morphology, frequency and tissue distribution distinguish perivascular cells from pericytes and intraparenchymal microglia. It is suggested that MHC class II positive perivascular cells are a normal constituent of the human cerebral microvasculature. The potential role of these cells in immunological reactions occurring at the blood-brain interface is discussed.
利用光镜和电镜免疫细胞化学技术，对手术切除及尸检的脑活检组织中主要组织相容性复合体（MHC）I类和II类抗原的表达进行了研究。此外，还应用了针对人巨噬细胞的单克隆抗体（EBM11）和α-平滑肌肌动蛋白。结果显示，在组织学上正常的人脑组织中，与血管相关的MHC II类免疫反应性可定位于一类独特的细胞，即血管周细胞，这类细胞具有巨噬细胞抗原，但不具有平滑肌细胞抗原。这种免疫表型、在血管周围间隙的位置以及形态、频率和组织分布，将血管周细胞与周细胞和实质内小胶质细胞区分开来。提示MHC II类阳性血管周细胞是人类脑微血管的正常组成部分。文中还讨论了这些细胞在血脑界面发生的免疫反应中的潜在作用。
