Rathore N, Kale M, John S, Bhatnagar D
School of Biochemistry, D. A. University, Indore.
Indian J Physiol Pharmacol. 2000 Apr;44(2):161-6.
Isoproterenol, upon oxidation, produces quinones which react with oxygen to produce superoxide anions (O2.-) and H2O2. In the present study, isoproterenol was administered to rats in two doses so as to evaluate its beta adrenergic and toxicological action in terms of lipid peroxidation (LPO) and antioxidant enzymes in erythrocytes. Isoproterenol (30 mg/100 g body wt.) was administered to rats and the animals were followed up to 7 days after administration. Some of these animals were treated with a second dose of isoproterenol 24 h after the first dose and the animals were followed up to 12 h. The result showed increased lipid peroxidation (LPO) and superoxide dismutase (SOD) activity in erythrocytes in response to isoproterenol. Catalase (CAT) activity in erythrocytes decreased with isoproterenol between day 2-7 as compared to control. The second injection of isoproterenol showed increased CAT activity in erythrocytes which decreased at 12 h as compared to control. The erythrocyte GSH content and glutathione-S-transferase (GST) activity decreased with isoproterenol treatment as compared to control. However, erythrocyte GSH content as well as GST activity both recovered towards control with time. Elevated serum lactate dehydrogenase (LDH), creatine phosphokinase (CPK) and glutamate oxaloacetate transaminase (GOT) activity was observed after isoproterenol treatment. The results show increased LPO and altered antioxidant system in erythrocytes in response to isoproterenol induced oxidative stress.
异丙肾上腺素氧化后会产生醌类物质，这些醌类物质与氧气反应生成超氧阴离子（O2.-）和过氧化氢（H2O2）。在本研究中，以两种剂量给大鼠注射异丙肾上腺素，以便从红细胞中的脂质过氧化（LPO）和抗氧化酶方面评估其β-肾上腺素能和毒理学作用。给大鼠注射异丙肾上腺素（30mg/100g体重），给药后对动物进行7天的跟踪观察。其中一些动物在首次给药后24小时接受第二次异丙肾上腺素给药，并对动物进行12小时的跟踪观察。结果显示，异丙肾上腺素会导致红细胞中的脂质过氧化（LPO）增加，超氧化物歧化酶（SOD）活性增强。与对照组相比，在第2至7天，异丙肾上腺素会使红细胞中的过氧化氢酶（CAT）活性降低。第二次注射异丙肾上腺素后，红细胞中的CAT活性增加，但与对照组相比，在12小时时降低。与对照组相比，异丙肾上腺素处理会使红细胞谷胱甘肽（GSH）含量和谷胱甘肽-S-转移酶（GST）活性降低。然而，随着时间的推移，红细胞GSH含量以及GST活性均恢复至对照水平。异丙肾上腺素处理后，血清乳酸脱氢酶（LDH）、肌酸磷酸激酶（CPK）和谷氨酸草酰乙酸转氨酶（GOT）活性升高。结果表明，异丙肾上腺素诱导的氧化应激会导致红细胞中LPO增加，抗氧化系统发生改变。
