Shervington Leroy A, Abba Michael, Hussain Bushra, Donnelly James
University of Central Lancashire, Department of Biological Sciences, Preston PR1 2HE, UK.
J Pharm Biomed Anal. 2005 Sep 15;39(3-4):769-75. doi: 10.1016/j.jpba.2005.04.039.
A rapid and reliable HPLC method was developed for the simultaneously separation and quantitation of five quinolones antibiotics; nalidixic acid, norfloxacin, ofloxacin, ciprofloxacin and lomefloxacin. All five tablet formulations of individual quinolone antibiotics were routinely assayed without interference. The calibration curves were linear (r2> or =0.999) over the concentration range of 1.20-4.8 mg/100 ml. Selectivity, precision, sensitivity and accuracy were established and the method is stability indicating with respect to ofloxacin. The limit of detection and quantitation for ofloxacin was 18 and 36 microg/100 ml, respectively. The separation was performed on a Phenomenex ODS C18 column using an isocratic, ion-pairing mobile phase consisting of 35% (v/v) aqueous acetonitrile together with tetrabutylammonium acetate, sodium dodecyl sulphate and citric acid (pH* 3.4). All analyses were conducted at ambient temperature and was monitored using a Diode Array UV/VIS detector set at wavelengths 235, 254, 275 and 300 nm.
建立了一种快速可靠的高效液相色谱法，用于同时分离和定量测定5种喹诺酮类抗生素：萘啶酸、诺氟沙星、氧氟沙星、环丙沙星和洛美沙星。对5种喹诺酮类抗生素的片剂制剂进行常规分析时均无干扰。校准曲线在1.20 - 4.8 mg/100 ml浓度范围内呈线性（r2≥0.999）。确定了选择性、精密度、灵敏度和准确度，该方法对氧氟沙星具有稳定性指示作用。氧氟沙星的检测限和定量限分别为18和36 μg/100 ml。分离在Phenomenex ODS C18柱上进行，使用等度离子对流动相，该流动相由35%（v/v）乙腈水溶液与醋酸四丁铵、十二烷基硫酸钠和柠檬酸（pH 3.4）组成。所有分析均在室温下进行，使用二极管阵列紫外/可见检测器在235、254、275和300 nm波长下进行监测。
