Matthews N, Maclaurin B P, Clarke G N
Aust J Exp Biol Med Sci. 1976 Oct;53(5):389-98. doi: 10.1038/icb.1975.44.
The normal human lymphocyte population which exhibits "spontaneous" cytolysis of EB2 Burkitt's lymphoma cells has been characterized. The effector cell has EA and EAC' receptors but lacks E receptors and probably surface Ig. "Spontaneous" anti-EB2 cytotoxicity was not reduced by preincubation of the effector cells with plastic or iron carbonyl or by passage through cotton wool or agarose columns but was reduced by passage through nylon wool columns. Thymocytes were not cytotoxic to EB2 cells, and chronic lymphocytic leukaemia cells (of B cell characteristics) had reduced cytotoxicity compared with normal lymphocytes. Cells from various lymphoid organs of rats and guinea-pigs were also cytotoxic to EB2 cells with reactivity in spleen greater than or equal to blood greater than lymph nodes. Spleen cells from neonatally thymectomized rats had increased cytotoxicity compared with normal rat spleen cells, suggesting that T lymphocytes are not essential. The effector cell in rat spleen did not adhere to cotton wool or agarose columns, indicating some resemblance to its counterpart in human peripheral blood.
已对表现出对EB2伯基特淋巴瘤细胞“自发”细胞溶解作用的正常人淋巴细胞群体进行了特性分析。效应细胞具有EA和EAC'受体，但缺乏E受体且可能缺乏表面免疫球蛋白。效应细胞与塑料或羰基铁预孵育、或通过棉绒或琼脂糖柱处理后，“自发”抗EB2细胞毒性并未降低，但通过尼龙棉柱处理后则降低。胸腺细胞对EB2细胞无细胞毒性，慢性淋巴细胞白血病细胞（具有B细胞特征）与正常淋巴细胞相比，细胞毒性降低。大鼠和豚鼠各种淋巴器官的细胞对EB2细胞也具有细胞毒性，脾脏中的反应性大于或等于血液大于淋巴结。新生期胸腺切除大鼠的脾细胞与正常大鼠脾细胞相比，细胞毒性增加，这表明T淋巴细胞并非必不可少。大鼠脾脏中的效应细胞不黏附于棉绒或琼脂糖柱，表明其与人类外周血中的对应细胞有一些相似之处。
