Matthews N, Rolland J M, Nairn R C
J Immunol Methods. 1976;9(3-4):323-35. doi: 10.1016/0022-1759(76)90207-6.
Fractionation by columns of aggregated rat immunoglobulin (Agg Ig)-agarose was investigated as a method of separating different populations of lymphoid cells. With rat spleen cells, Agg Ig columns retained phagocytes, IgM- and IgG-antibody-forming-cells, cells mediating antibody- or PHA-induced lysis of chicken erythrocytes, and specifically immune splenocytes lytic to chicken erythrocytes without exogenous antibody. Agg Ig columns did not selectively remove 'B lymphocytes' (surface-Ig-bearing lymphocytes with or without EAC' receptors), or T lymphocytes capable of PHA-induced proliferation or graft-versus-host reactivity. With mouse spleen cells, Agg Ig columns retained alloimmune cytotoxic T cells.
研究了用聚集大鼠免疫球蛋白（Agg Ig）-琼脂糖柱进行分级分离，作为一种分离不同淋巴细胞群体的方法。对于大鼠脾细胞，Agg Ig柱保留吞噬细胞、IgM和IgG抗体形成细胞、介导抗体或PHA诱导的鸡红细胞裂解的细胞，以及无需外源性抗体即可特异性免疫裂解鸡红细胞的脾细胞。Agg Ig柱不会选择性去除“B淋巴细胞”（带有或不带有EAC受体的表面免疫球蛋白阳性淋巴细胞），或能够进行PHA诱导增殖或移植物抗宿主反应的T淋巴细胞。对于小鼠脾细胞，Agg Ig柱保留同种免疫细胞毒性T细胞。
